Sci Rep:研究进一步揭示miR-16-5p与肠道病毒71之间的作用关系

2017-12-05 MedSci MedSci原创

肠道病毒71(EV71)是手足口病(HFMD)的主要病原体。与其他引起手足口病的肠道病毒不同,EV71会导致严重的神经系统并发症,甚至死亡。微小RNAs(miRNAs)是小的非编码RNAs,参与了许多生物或病理过程。我们之前报道,通过研究手足口病患者中宿主miRNA的表达模式,发现miR-16-5p的表达水平会随着手足口病的严重程度而增加。然而,EV71诱导miR-16-5p表达的机制尚不清楚,E

肠道病毒71(EV71)是手足口病(HFMD)的主要病原体。与其他引起手足口病的肠道病毒不同,EV71会导致严重的神经系统并发症,甚至死亡。微小RNAs(miRNAs)是小的非编码RNAs,参与了许多生物或病理过程。

我们之前报道,通过研究手足口病患者中宿主miRNA的表达模式,发现miR-16-5p的表达水平会随着手足口病的严重程度而增加。然而,EV71诱导miR-16-5p表达的机制尚不清楚,EV71和miR-16-5p之间的相互作用也并不完全清楚。

本研究中,我们探究了EV71诱导的miR-16-5p在体外和体内的表达,发现EV71感染后miR-16-5p的上调可能发生在转录后水平。而且,EV71诱导的胱天蛋白酶活化促进了pri-miR-16-1的上调。此外,我们还发现miR-16-5p可以通过激活caspase-3促进EV71诱导的神经细胞凋亡,而miR-16-5p可以抑制EV71的复制。CCNE1和CCND1是两个重要的细胞周期调控因子,在miR-16-5p抑制EV71复制中发挥重要作用。因此,miR-16-5p是EV71诱导的细胞凋亡和病毒复制抑制剂的正反馈调节因子。

综上所述,该研究结果有助于理解miRNA与EV71感染之间的相互作用网络,并为开发抗病毒治疗提供新的潜在靶点。

原始出处:

Caishang Zheng,1,2 Zhenhua Zheng, et al., MiR-16-5p mediates a positive feedback loop in EV71-induced apoptosis and suppresses virus replication. Sci Rep. 2017; 7: 16422. doi:  10.1038/s41598-017-16616-7

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    2018-01-21 smallant2002
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    2017-12-08 1e145228m78(暂无匿称)

    学习了谢谢作者分享!

    0

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    2017-12-06 1dd8c52fm63(暂无匿称)

    学习学习.继续关注

    0

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    2017-12-06 飛歌

    学习了很有用

    0

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