如何设计高水平的基础研究课题

2015-07-08 MedSci MedSci原创

研究类型与循证医学证据Produced by Bob Phillips, Chris Ball, Dave Sackett, Doug Badenoch, Sharon Straus, Brian Haynes, Martin Dawes since November 1998.由牛津大学循证医学中心制定，www.cebm.net基础研究是临床研究的“奠基石”，适合对基因和药物分子的分子机制进行纵

研究类型与循证医学证据

Produced by Bob Phillips, Chris Ball, Dave Sackett, Doug Badenoch, Sharon Straus, Brian Haynes, Martin Dawes since November 1998.由牛津大学循证医学中心制定，www.cebm.net

基础研究是临床研究的“奠基石”，适合对基因和药物分子的分子机制进行纵深研究。基础研究的目的在于解决一个实际的基础或临床方面问题；相对于临床研究来说，它对于创新性的要求更高。研究设计同时要考虑其可行性和完整性。

基础研究创新的几条途径

日常线索法：日常的实验工作中要做一个“有心人”，保持敏感的科研“嗅觉”。可在实验室已有的研究基础上进行创新。

文献指摘法：比如对研究中只有表型缺少机制的，可以将机制细化寻求创新。

综述梳理法：梳理整个领域的发展脉络，找出领域将来发展的轨迹。比如，对于骨髓间充质干细胞（MSC）移植研究，可以梳理为分化论、营养论、免疫论。只有对科研进展有全面的认识，才能正确的选题。

基因筛选法：包括酵母双杂交或蛋白质pull－down联合质谱分析寻找关键蛋白的相互作用分子、PB转座子基因筛选系统、全基因组siRNA筛选系统和组学分析筛选系统。

基础研究创新途径很多，关键是找到适合自己的。往往创新性要求高的课题，可行性难度也高。

基础研究四个要素

研究对象：研究对象的创新是最难的！往往需要对该领域有一个全局观和一定的灵感。可以使用文献分析法和组学筛选法。文献分析法：“门槛”较高，容易“撞车”；组学分析法： “门槛”不高，只要你有钱能“任性”。

研究系统：

研究系统的适用性

研究系统维创新的关键是在不同的研究系统间进行切换，原则是从低等向高等进行升级。

研究手段：比如热点实验技术：iPSCs重编程，基因编辑等；定量检测技术。

研究机制：现象>>机理研究与分析>>结论

研究设计思路要与实验方法相结合。基础研究的重点可以是新的机制，也可以是机制在不同系统中的验证，不同机制之间的联系。

总之，基础研究的选题首先要了解研究的大方向，寻找没有明确结论的问题，缩小研究方向。也可以换个角度找答案，增加研究的创新性。

相关课程请登陆MedSci学院观看：

http://edu.medsci.cn/course_info/medsci-ji-chu-yan-jiu-chuang-xin.html

或下载MedSci医学app，观看课程免一半积分。更多新推课程免积分观看！

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2017-12-17 huizi3382

还是需要慢慢摸索

65 0
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2016-03-17 忠诚向上

好好学习

103 0
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2016-03-17 忠诚向上

好好学习

109 0
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2016-03-17 忠诚向上

好好学习

144 0
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2016-03-17 忠诚向上

好好学习

113 0
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2016-03-17 忠诚向上

好好学习

71 0
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2016-03-17 忠诚向上

好好学习

85 0
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2016-03-17 忠诚向上

好好学习

104 0
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2016-03-17 忠诚向上

好好学习

82 0
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2016-03-17 忠诚向上

好好学习

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