PLoS ONE:挥发性麻醉药物对巨噬细胞吞噬作用的影响

2019-09-30 anesthGH “罂粟花”微信号

围手术期感染,特别是手术部位感染引起显着的发病率和死亡率。吞噬作用是去除微生物的关键步骤。我们检测了常用麻醉药对巨噬细胞吞噬作用的影响及其机制。

背景与目的

围手术期感染,特别是手术部位感染引起显着的发病率和死亡率。吞噬作用是去除微生物的关键步骤。我们检测了常用麻醉药对巨噬细胞吞噬作用的影响及其机制。

方  法

采用RAW264.7小鼠细胞,小鼠腹腔巨噬细胞和THP-1人细胞检测麻醉药(异氟醚,七氟醚,异丙酚)对巨噬细胞吞噬功能的影响。将受调理的绵羊红细胞或荧光标记的大肠杆菌用作吞噬细胞。Rap1是吞噬作用中的关键蛋白,使用活性Rap1和检测试剂盒评估Rap1的活化。使用azi-异氟烷和azi-七氟醚进行光学标记实验监测Rap1上的麻醉剂结合位点。以Rap1的丙氨酸扫描以评估麻醉剂结合位点在Rap1活化和吞噬作用中的诱变作用。

结 果

异氟醚和七氟醚暴露后,巨噬细胞吞噬功能明显减弱,而异丙酚则没有(1%异氟醚减少50%,1.5%七氟醚减少50%)。光标记实验表明,七氟醚直接与Rap1结合。诱变分析表明,七氟醚结合位点影响Rap1活化和巨噬细胞吞噬作用。

结 论

我们发现异氟醚和七氟醚减弱了巨噬细胞的吞噬作用,但异丙酚却没有。

原始出处:

Zha Hui,Matsunami Erika,Blazon-Brown Nathan et al. Volatile anesthetics affect macrophage phagocytosis.[J] .PLoS ONE, 2019, 14: e0216163.

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    2019-09-30 医者仁心5538

    学习了

    0