ACS Appl Mater Interfaces:新型功能化的电纺丝素蛋白支架可有效促进骨修复

2018-03-17 MedSci MedSci原创

研发可改善成骨能力的功能性支架对于骨组织工程中的骨形成和矿化十分重要。在这项研究中,我们开发了两阶段羟磷灰石(HAp)粒子功能化的电纺丝素蛋白(SF)纳米纤维支架,使用多巴胺(PDA)粘附。在静电纺丝过程中首先将HAp颗粒结合到SF支架中,然后通过PDA将其固定在含有HAp的电纺SF纳米纤维支架上。我们获得了两阶段HAp功能化的SF纳米纤维支架,机械性能优良,并能够提供骨特异性生理微环境。对所开发

研发可改善成骨能力的功能性支架对于骨组织工程中的骨形成和矿化十分重要。在这项研究中,我们开发了两阶段羟磷灰石(HAp)粒子功能化的电纺丝素蛋白(SF)纳米纤维支架,使用多巴胺(PDA)粘附。

在静电纺丝过程中首先将HAp颗粒结合到SF支架中,然后通过PDA将其固定在含有HAp的电纺SF纳米纤维支架上。我们获得了两阶段HAp功能化的SF纳米纤维支架,机械性能优良,并能够提供骨特异性生理微环境。

对所开发的支架进行体外增强人脂肪源间充质干细胞(hADMSCs)的成骨分化和体内修复骨缺损的能力测试。为了提高它们的骨修复能力,我们通过聚合物纳米颗粒介导的基因递送将具有PDZ-结合基序(TAZ)的转录共激活因子基因修饰hADMSCs。众所周知,TAZ是一种转录调节剂,可激活间充质干细胞(MSC)的成骨分化。结果显示,两阶段HAp功能化的SF支架在体外可明显促进TAZ转染的hADMSCs的成骨分化并增强了临界尺寸颅骨骨缺损模型中的矿化骨形成。

总之,该研究结果表明,具有纳米纤维结构并富集无机组分的SF支架在骨组织工程中的潜在用途。

原始出处:


Ko E, Lee JS, et al., Electrospun Silk Fibroin Nanofibrous Scaffolds with Two-Stage Hydroxyapatite Functionalization for Enhancing the Osteogenic Differentiation of Human Adipose-Derived Mesenchymal StemCells. ACS Appl Mater Interfaces. 2018 Mar 7;10(9):7614-7625. doi: 10.1021/acsami.7b03328. Epub 2017 May 5.

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    2018-12-12 shanyongle
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    2018-04-28 axin020
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    2018-03-19 yaanren