日采用新iPS细胞技术治疗糖尿病

2015-02-17 新华 新华网

京都大学教授长船健二率领的研究小组日前宣布,他们利用人类诱导多功能干细胞(iPS细胞),高效制作出了能够发育成胰腺细胞的胰芽(pancreatic bud)细胞,移植到实验鼠体内后,确认能正常发挥作用。这一发现有望促进开发出治疗糖尿病的方法。 iPS细胞是体细胞经诱导因子处理后转化而成的干细胞,其功能与胚胎干细胞类似,能发育成多种组织和器官。 胰芽细胞能够发育成分泌胰岛素的

京都大学教授长船健二率领的研究小组日前宣布,他们利用人类诱导多功能干细胞(iPS细胞),高效制作出了能够发育成胰腺细胞的胰芽(pancreatic bud)细胞,移植到实验鼠体内后,确认能正常发挥作用。这一发现有望促进开发出治疗糖尿病的方法。

iPS细胞是体细胞经诱导因子处理后转化而成的干细胞,其功能与胚胎干细胞类似,能发育成多种组织和器官。

胰芽细胞能够发育成分泌胰岛素的β细胞等胰腺的各种细胞。虽然此前也有研究人员报告了几种将iPS细胞培育为胰芽细胞的方法,但是由于没有充分弄清机制,效率依然较低。

研究小组注意到,称为“胰腺前驱细胞”的单层细胞片形成胰芽细胞的团块后,才会出现胰腺的形状,为此,他们利用iPS细胞培养胰芽细胞时,不是在培养皿中进行平面培养,而是让胰腺前驱细胞漂浮在培养液中,使其成为团块状,制作出如同胰腺在胎儿体内发育的环境,然后添加3种促进分化的蛋白质,最终团块中约40%的胰腺前驱细胞发育成了胰芽细胞。而如果在培养皿中进行平面培养,最多也只有不到20%的胰腺前驱细胞能够转化为胰芽细胞。

研究小组将培育出的胰芽细胞移植给十几只实验鼠后,发现这些胰芽细胞扎下根来,形成了类似胎儿胰腺的组织结构,最终分化出了能够根据血糖值上升而分泌胰岛素的成熟胰腺β细胞。

长船健二指出:“今后准备改良培养方法,进一步提高效率并削减制作费用,在5至10年内开展移植给人类的临床研究。”


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  1. [GetPortalCommentsPageByObjectIdResponse(id=20380, encodeId=296820380f0, content=AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20150920/IMG55FDE671A89482379.jpg, createdBy=cef3108336, createdName=x35042875, createdTime=Sat Apr 11 22:46:00 CST 2015, time=2015-04-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16649, encodeId=81c61664951, content=非常好的文章,学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Sat Feb 21 10:44:00 CST 2015, time=2015-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16601, encodeId=428d166016a, content=很不错学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Fri Feb 20 13:27:00 CST 2015, time=2015-02-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1377720, encodeId=d6d313e77209c, content=<a href='/topic/show?id=17b810004ce' target=_blank style='color:#2F92EE;'>#iPS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10004, encryptionId=17b810004ce, topicName=iPS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69cb413, createdName=chengjn, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527114, encodeId=5e8e152e114c4, content=<a href='/topic/show?id=24d610019a8' target=_blank style='color:#2F92EE;'>#iPS细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10019, encryptionId=24d610019a8, topicName=iPS细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a27a11802753, createdName=wmr115, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=)]
    2015-04-11 x35042875

    AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=20380, encodeId=296820380f0, content=AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20150920/IMG55FDE671A89482379.jpg, createdBy=cef3108336, createdName=x35042875, createdTime=Sat Apr 11 22:46:00 CST 2015, time=2015-04-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16649, encodeId=81c61664951, content=非常好的文章,学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Sat Feb 21 10:44:00 CST 2015, time=2015-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16601, encodeId=428d166016a, content=很不错学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Fri Feb 20 13:27:00 CST 2015, time=2015-02-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1377720, encodeId=d6d313e77209c, content=<a href='/topic/show?id=17b810004ce' target=_blank style='color:#2F92EE;'>#iPS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10004, encryptionId=17b810004ce, topicName=iPS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69cb413, createdName=chengjn, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527114, encodeId=5e8e152e114c4, content=<a href='/topic/show?id=24d610019a8' target=_blank style='color:#2F92EE;'>#iPS细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10019, encryptionId=24d610019a8, topicName=iPS细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a27a11802753, createdName=wmr115, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=)]
    2015-02-21 orthoW

    非常好的文章,学习

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=20380, encodeId=296820380f0, content=AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20150920/IMG55FDE671A89482379.jpg, createdBy=cef3108336, createdName=x35042875, createdTime=Sat Apr 11 22:46:00 CST 2015, time=2015-04-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16649, encodeId=81c61664951, content=非常好的文章,学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Sat Feb 21 10:44:00 CST 2015, time=2015-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16601, encodeId=428d166016a, content=很不错学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Fri Feb 20 13:27:00 CST 2015, time=2015-02-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1377720, encodeId=d6d313e77209c, content=<a href='/topic/show?id=17b810004ce' target=_blank style='color:#2F92EE;'>#iPS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10004, encryptionId=17b810004ce, topicName=iPS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69cb413, createdName=chengjn, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527114, encodeId=5e8e152e114c4, content=<a href='/topic/show?id=24d610019a8' target=_blank style='color:#2F92EE;'>#iPS细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10019, encryptionId=24d610019a8, topicName=iPS细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a27a11802753, createdName=wmr115, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=)]
    2015-02-20 orthoW

    很不错学习了

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=20380, encodeId=296820380f0, content=AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20150920/IMG55FDE671A89482379.jpg, createdBy=cef3108336, createdName=x35042875, createdTime=Sat Apr 11 22:46:00 CST 2015, time=2015-04-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16649, encodeId=81c61664951, content=非常好的文章,学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Sat Feb 21 10:44:00 CST 2015, time=2015-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16601, encodeId=428d166016a, content=很不错学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Fri Feb 20 13:27:00 CST 2015, time=2015-02-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1377720, encodeId=d6d313e77209c, content=<a href='/topic/show?id=17b810004ce' target=_blank style='color:#2F92EE;'>#iPS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10004, encryptionId=17b810004ce, topicName=iPS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69cb413, createdName=chengjn, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527114, encodeId=5e8e152e114c4, content=<a href='/topic/show?id=24d610019a8' target=_blank style='color:#2F92EE;'>#iPS细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10019, encryptionId=24d610019a8, topicName=iPS细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a27a11802753, createdName=wmr115, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=)]
    2015-02-19 chengjn
  5. [GetPortalCommentsPageByObjectIdResponse(id=20380, encodeId=296820380f0, content=AKI对心衰临床治疗意义重大,但依然需要研究检验其适用性, beContent=null, objectType=article, channel=null, level=null, likeNumber=114, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://cacheapi.medsci.cn/resource/upload/20150920/IMG55FDE671A89482379.jpg, createdBy=cef3108336, createdName=x35042875, createdTime=Sat Apr 11 22:46:00 CST 2015, time=2015-04-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16649, encodeId=81c61664951, content=非常好的文章,学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=143, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Sat Feb 21 10:44:00 CST 2015, time=2015-02-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=16601, encodeId=428d166016a, content=很不错学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=140, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=f2fb1607679, createdName=orthoW, createdTime=Fri Feb 20 13:27:00 CST 2015, time=2015-02-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1377720, encodeId=d6d313e77209c, content=<a href='/topic/show?id=17b810004ce' target=_blank style='color:#2F92EE;'>#iPS#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10004, encryptionId=17b810004ce, topicName=iPS)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=69cb413, createdName=chengjn, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1527114, encodeId=5e8e152e114c4, content=<a href='/topic/show?id=24d610019a8' target=_blank style='color:#2F92EE;'>#iPS细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=10019, encryptionId=24d610019a8, topicName=iPS细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=a27a11802753, createdName=wmr115, createdTime=Thu Feb 19 01:17:00 CST 2015, time=2015-02-19, status=1, ipAttribution=)]
    2015-02-19 wmr115

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