JCC:肠系膜淋巴结微生物组成可以区分克罗恩病和溃疡性结肠炎

2018-10-05 MedSci MedSci原创

肠系膜淋巴结是易位细菌介导炎症发生并引起免疫反应的关键部位。因此,了解炎症性肠病中肠系膜淋巴结的微生物组成很重要。本研究旨在研究克罗恩病和溃疡性结肠炎患者的肠系膜淋巴结微生物群之间的差异。

背景及目标
肠系膜淋巴结是易位细菌介导炎症发生并引起免疫反应的关键部位。因此,了解炎症性肠病中肠系膜淋巴结的微生物组成很重要。本研究旨在研究克罗恩病溃疡性结肠炎患者的肠系膜淋巴结微生物群之间的差异。

方法
研究人员从克罗恩病和经历切除的溃疡性结肠炎患者中收集肠系膜淋巴结标本。从肠系膜淋巴结中提取总DNA并评估细菌DNA(16S)的存在。对16S DNA呈阳性的样品进行下一代测序,并测定细菌的组成成分。

结果
研究人员发现克罗恩病肠系膜淋巴结与溃疡性结肠炎的淋巴结中观察到的微生物特征明显不同。溃疡性结肠炎患者淋巴结中Firmicutes菌的相对丰度较大,而克罗恩病患者中Proteobacteria菌的丰度较高。虽然克罗恩病肠系膜淋巴结的物种多样性减少,但它们含有更多的不太常见的菌种,占主要成分的是梭杆菌。

结论
该研究证实克罗恩病与溃疡性结肠炎肠系膜淋巴结微生物组之间存在明显差异。这种微生物差异可以帮助诊断克罗恩病或溃疡性结肠炎,特别是在切除时不确定性结肠炎时。

原始出处:

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    2019-04-21 nymo
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    2018-10-07 sodoo
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    2018-10-07 zzc2216
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    2018-10-05 liumin1987

    谢谢分享,学习了。

    0

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