Circulation:C-Kit+来源的内皮细胞Gata4依赖性的细胞分化揭露了心肌细胞再生的假象!

2018-09-10 MedSci MedSci原创

既往报道在心脏中,c-Kit+成人祖细胞可形成新的心肌细胞,但近期有遗传证据表明这种情况非常罕见。这种罕见事件是否可代表真正的新生心肌细胞形成?现有研究人员对此进行研究。Bryan D. Maliken等人建立了特异性敲除c-Kit+心肌祖细胞的必要心源性转录因子Gata4和Gata6。在双敲小鼠中进行Kit等位基因依赖性谱系追踪和融合分析,来检测c-Kit+细胞形成新生心肌细胞的发生率。同时采用

既往报道在心脏中,c-Kit+成人祖细胞可形成新的心肌细胞,但近期有遗传证据表明这种情况非常罕见。这种罕见事件是否可代表真正的新生心肌细胞形成?现有研究人员对此进行研究。

Bryan D. Maliken等人建立了特异性敲除c-Kit+心肌祖细胞的必要心源性转录因子Gata4和Gata6。在双敲小鼠中进行Kit等位基因依赖性谱系追踪和融合分析,来检测c-Kit+细胞形成新生心肌细胞的发生率。同时采用骨髓移植试验鉴定Kit等位基因-衍生的血液细胞比对心脏内源性的Kit谱系依赖性细胞对新生谱系示踪的心肌细胞的贡献度。

敲除Kit谱系来源的内皮细胞或全内皮细胞的Gata4,但不编辑骨髓细胞,会导致内皮细胞明显扩张和分化缺陷、白细胞浸润心脏、Kit等位基因依赖性谱系示踪的心肌细胞增多。但是,这种标记的心肌细胞增加是白细胞-心肌细胞融合的假象,因为在缺乏Gata4的情况下,内皮细胞不能正常分化。

以前采用Kit等位基因谱系追踪发现在c-Kit细胞在心脏中可形成新生心肌细胞,似乎是标记的白细胞与心肌细胞融合的假象。敲除c-Kit+内皮祖细胞或成人内皮细胞的Gata4,对血管生成和毛细血管网完整性产生负性作用。

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    2018-09-12 bbjsj_1981
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    2018-09-12 shuangle