Nature:声学报告基因让超声波为人体内的细菌成像

2018-01-08 药明康德 学术经纬

哺乳动物的微生物组在生物健康和疾病方面有很多重要作用,而且基因工程让开发基于微生物的疗法和诊断方法成为可能。对于了解天然或基因工程改造后的微生物在宿主体内的活性来说首要的一点是确定它们在宿主体内的位置。

哺乳动物的微生物组在生物健康和疾病方面有很多重要作用,而且基因工程让开发基于微生物的疗法和诊断方法成为可能。对于了解天然或基因工程改造后的微生物在宿主体内的活性来说首要的一点是确定它们在宿主体内的位置。但是目前对细胞位置和功能的成像技术大多依靠光学报告基因(optic reporter gene),由于光散射的原因,这些报告基因在深度组织中的表现欠佳。加州理工学院 (California Institute of Technology) 的研究人员开发出一种声学报告基因 (acoustic reporter gene),这些报告基因让表达它们的细菌可以被超声波成像,而超声波具备很好的深度组织穿透能力和很高的空间分辨率。某些水生的光合生物需要表达一种称为微型气泡的气体填充的蛋白纳米结构来调节浮力,声学报告基因就是基于这些微型气泡设计而成的。当大肠杆菌(Escherichia coli)或鼠伤寒沙门氏菌(Salmonella typhimurium)表达生成微型气泡的一系列基因后,使用超声波可以在体积密度小于0.01%的情况下对它们进行成像,分辨率达到100微米以下。研究人员不但在胃肠道模型和癌症模型中成功检测到细菌在宿主体内的位置,而且通过开发具备不同声学特征的报告基因,他们可以对多种细胞同时进行成像。这项技术将促进对哺乳动物微生物组的研究,并且有助于开发用于诊断和治疗的细胞疗法。

原始出处:

Raymond W. Bourdeau,et al.,Acoustic reporter genes for noninvasive imaging of microorganisms in mammalian hosts.Nature.553, 86–90 (04 January 2018).

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    2018-03-21 liye789132251
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    2018-07-27 yyj074
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    2018-01-08 戒馋,懒,贪

    谢谢分享学习了

    0