Hum Gene Ther:hWnt10b可以加速种植体周围的骨结合

2018-06-23 MedSci MedSci原创

本研究旨在探讨人Wnt10b(hWnt10b)转基因加速卵巢切除(OVX)大鼠钛种植体周围骨整合的疗效,为骨质疏松症患者的种植治疗提供新的策略。体内建立双侧卵巢切除的骨质疏松大鼠模型,观察Wnt通路相关基因的表达变化。然后将Ad-hWnt10b在植入OVX大鼠的股骨缺损之前局部使用,植入后分别在植入后1周和3周收集周围股骨组织以用于成像,生物力学测试,分子和组织学分析。在体外,使用含有hWnt10

本研究旨在探讨人Wnt10b(hWnt10b)转基因加速卵巢切除(OVX)大鼠钛种植体周围骨整合的疗效,为骨质疏松症患者的种植治疗提供新的策略。

体内建立双侧卵巢切除的骨质疏松大鼠模型,观察Wnt通路相关基因的表达变化。然后将Ad-hWnt10b在植入OVX大鼠的股骨缺损之前局部使用,植入后分别在植入后1周和3周收集周围股骨组织以用于成像,生物力学测试,分子和组织学分析。在体外,使用含有hWnt10b的腺病毒(Ad-hWnt10b)转染的骨髓基质细胞(BMSCs)分别用脂肪生成和成骨诱导培养基培养2周。进行茜素红染色,油红O染色,RT-PCR和Western印迹分析以评估hWnt10b对BMSCs分化的影响。

结果显示,骨质疏松大鼠模型中Wnt通路明显下调。Ad-hWnt10b转染OVX大鼠股骨缺损后,ALP、Runx-2和骨钙素表达明显增加,组织蛋白酶K的表达降低。组织学和成像分析显示,Ad-hWnt10b组骨形成蛋白阳性细胞的数量以及新形成的骨的密度显著高于Ad-hWnt10b组。同时,在体外,Ad-hWnt10b-BMSCs的成骨明显增加,脂肪生成明显减少。

综上所述,该研究结果表明,hWnt10b可以加速种植体周围的骨结合,并随后增强OVX条件下的骨再生和种植体稳定性。

原始出处:

Liu H, Zhang N, et al., Effect of hWnt10b Transgene Overexpression on Peri-implant Osteogenesis in Ovariectomized Rats. Hum Gene Ther. 2018 May 23. doi: 10.1089/hum.2018.003.

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    2018-10-29 cy0324
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    2019-02-07 仁医06
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    2018-06-25 zhwj