J Endod:粪肠球菌可以水解牙科用树脂复合物及粘接剂

2018-02-08 lishiting MedSci原创

在根管治疗术后,牙本质-封闭剂界面会发生降解,导致界面菌斑生物膜增殖和治疗失败。唾液和致龋菌表现出脂酶样的活性(如:胆固醇脂酶[CE]样或/和拟胆碱酯酶[PCE]样),它会降解甲基丙烯酸酯类树脂材料和/或修复体-牙齿界面,进而增加界面微生物的增殖。粪肠球菌为革兰氏阳性细菌,它通常检出于持久感染的牙髓中。这篇研究的目的是为了检测粪肠球菌脂酶样、CE样和PCE样的活性,并评估细菌降解甲基丙烯酸酯类树脂

在根管治疗术后,牙本质-封闭剂界面会发生降解,导致界面菌斑生物膜增殖和治疗失败。唾液和致龋菌表现出脂酶样的活性(如:胆固醇脂酶[CE]样或/和拟胆碱酯酶[PCE]样),它会降解甲基丙烯酸酯类树脂材料和/或修复体-牙齿界面,进而增加界面微生物的增殖。粪肠球菌为革兰氏阳性细菌,它通常检出于持久感染的牙髓中。这篇研究的目的是为了检测粪肠球菌脂酶样、CE样和PCE样的活性,并评估细菌降解甲基丙烯酸酯类树脂复合物以及全酸蚀和自酸蚀粘接剂的能力。研究分别采用硝基甲苯和丁酰硫胆碱底物检测粪肠球菌的CE样和PCE样活性。通过高效液相色谱法定量化通用树脂降解副产物的释放(如:双[羟丙氧基]-苯丙烷[BisHPPP])来检测粪肠球菌降解树脂复合物以及全酸蚀和自酸蚀粘接剂的能力。结果显示,粪肠球菌展现出CE样(1.23 ± 0.13 U/μg dry bacteria)而非PCE样活性。培养30天和/或14天后,BisHPPP的释放量在有细菌的培养基中要显著高于TE和RC培养基,但不是SE培养基(P < .05)。存在细菌的TE培养基培养30天后,BisHPPP的释放量最高(23.69 ± 1.72

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    2018-02-10 xzw115
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    2018-02-10 chenshujs

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