MJ:评估纯化间日疟原虫感染红细胞的NWF过滤器

2017-06-21 MedSci MedSci原创

在离体药物测定,体外侵袭测定以及基因组测序实验过程中常常需要从临床血液样品中分离疟原虫感染的红细胞(iRBC)。目前用于从疟疾感染的血液中去除白细胞(WBC)的方法比较耗时且费用昂贵的。本研究开发了一种用于纯化iRBC的原型无纺布(NWF)过滤器,且在试验研究中显示出很好的去除WBC的能力。先前的研究工作是通过优化处理5-10毫升血液的原型过滤器进行的。随着过滤器的不断商品化,本项研究的目的是评估

在离体药物测定,体外侵袭测定以及基因组测序实验过程中常常需要从临床血液样品中分离疟原虫感染的红细胞(iRBC)。目前用于从疟疾感染的血液中去除白细胞(WBC)的方法比较耗时且费用昂贵的。本研究开发了一种用于纯化iRBC的原型无纺布(NWF)过滤器,且在试验研究中显示出很好的去除WBC的能力。先前的研究工作是通过优化处理5-10毫升血液的原型过滤器进行的。随着过滤器的不断商品化,本项研究的目的是评估商业化NWF过滤器的效率和在更小体积血液净化间日疟原虫感染的红细胞的适用性,并与Plasmodipur®过滤器比较其性能的优劣。

从中国-缅甸边境疟疾门诊有症状的患者中采集43临床间日疟血液样本,并使用NWF过滤器在附近的现场实验室进行处理。比较了NWF过滤前后血液样品中白细胞和iRBC的数量以及间日疟原虫的形态。过滤后间日疟原虫的活力通过从27个血液样品体外短期培养进行测定。此外,在6份间日疟原虫血液样本对NWF过滤器去除白细胞的的有效性与Plasmodipur®进行比较。

NWF过滤器过滤的1-2毫升的感染的液WBC的去除率达到了99.68%。 过滤前后总iRBC,环状体和滋养体阶段的密度无著着性差异(P > 0.05)。然而,临床样本中较小的原虫阶段的裂殖体和配子体感染的红细胞的恢复率相对较低。过滤后,间日疟原虫没有显示明显的形态变化。对过滤后的27份间日疟血液样本进行培养显示它们均可以成功地进入裂殖体阶段。NWFPlasmodipur®过滤器的白细胞去除率和iRBC回收率方面不具有显著性差异(P > 0.05)。

当用1-2mL 间日疟原虫感染的血液进行测试时,NWF过滤器可以有效去除白细胞,对红细胞内环状体和滋养体的回收率较高。经过滤的间日疟原虫可以在体外成功培养至成熟。NWF Plasmodipur®在去除白细胞和回收iRBC的性能上可以相媲美。

原始出处:

Jiangyan LiZhiyong TaoQian LiAwtum Brashear,et.al. Further evaluation of the NWF filter for the purification of Plasmodium vivax-infected erythrocytes.

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    2017-06-22 stupidox

    学习了,很好。

    0

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