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Clinica Chimica Acta：采用液相色谱-串联质谱联用技术同时测定48种血浆氨基酸研究尿素循环紊乱

2019-09-22 gladiator MedSci原创

液相色谱-串联质谱联用
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尿素循环障碍(UCD)是一种先天的氨解毒错误，早期诊断和治疗是预防代谢异常的关键。遗憾的是，诊断经常被延迟。为了提高诊断水平，我们建立了一种液相色谱-串联质谱联用方法来研究UCD对氨基酸谱的干扰。

尿素循环障碍(UCD)是一种先天的氨解毒错误，早期诊断和治疗是预防代谢异常的关键。遗憾的是，诊断经常被延迟。为了提高诊断水平，我们建立了一种液相色谱-串联质谱联用方法来研究UCD对氨基酸谱的干扰。

新方法可以实现)在20 分钟完成48个氨基酸的绝对量化，并且只需要只有2.5 μL血浆样本。对于大多数AAs的下限为0.01 μmol / L。方法的准确度为89.9% ~ 113.4%。运行内、运行间变异系数分别为0.8-7.7%和2.6-14.5%。该方法通过对正常对照组150份样本的分析，为42份AAs建立了年龄特异性参考值，成功区分出不同亚型的UCD患者。患者资料显示，UCD不仅干扰尿素循环AAs的代谢，诱导氨排毒AAs的积累，还干扰pipecolic acid、N-acetylaspartic acid等神经系统相关AAs的代谢。

这一数据可能为UCD的发病机制提供新的见解。

原始出处：

Min-Zhi Peng，Yan-Na Cai，Simultaneous quantification of 48 plasma amino acids by liquid chromatography-tandem mass spectrometry to investigate urea cycle disorders

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    2019-11-07 zhaohui6731

    #氨基酸#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1860864, encodeId=b876186086451, content=<a href='/topic/show?id=5e746403ed5' target=_blank style='color:#2F92EE;'>#氨基酸#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64037, encryptionId=5e746403ed5, topicName=氨基酸)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bf3d82, createdName=zhaohui6731, createdTime=Thu Nov 07 12:05:00 CST 2019, time=2019-11-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849749, encodeId=0c501849e4954, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Mon Nov 11 03:05:00 CST 2019, time=2019-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662304, encodeId=659416623046a, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db925396819, createdName=丁鹏鹏, createdTime=Mon Apr 27 01:05:00 CST 2020, time=2020-04-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553366, encodeId=732b15533662f, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Tue Sep 24 08:05:00 CST 2019, time=2019-09-24, status=1, ipAttribution=)]
    2019-11-11 windight

    #CTA#

    0

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    2020-04-27 丁鹏鹏

    #测定#

    0

  4. [GetPortalCommentsPageByObjectIdResponse(id=1860864, encodeId=b876186086451, content=<a href='/topic/show?id=5e746403ed5' target=_blank style='color:#2F92EE;'>#氨基酸#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=64037, encryptionId=5e746403ed5, topicName=氨基酸)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=bf3d82, createdName=zhaohui6731, createdTime=Thu Nov 07 12:05:00 CST 2019, time=2019-11-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1849749, encodeId=0c501849e4954, content=<a href='/topic/show?id=61815385aa' target=_blank style='color:#2F92EE;'>#CTA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5385, encryptionId=61815385aa, topicName=CTA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ec9955, createdName=windight, createdTime=Mon Nov 11 03:05:00 CST 2019, time=2019-11-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1662304, encodeId=659416623046a, content=<a href='/topic/show?id=6a426545472' target=_blank style='color:#2F92EE;'>#测定#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65454, encryptionId=6a426545472, topicName=测定)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db925396819, createdName=丁鹏鹏, createdTime=Mon Apr 27 01:05:00 CST 2020, time=2020-04-27, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1553366, encodeId=732b15533662f, content=<a href='/topic/show?id=81bd923e559' target=_blank style='color:#2F92EE;'>#质谱#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=92375, encryptionId=81bd923e559, topicName=质谱)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=96fd14366106, createdName=jeanqiuqiu, createdTime=Tue Sep 24 08:05:00 CST 2019, time=2019-09-24, status=1, ipAttribution=)]
    2019-09-24 jeanqiuqiu

    #质谱#

    0