J Vis Exp:通过倾斜扫描激光检测眼睛电镜(oSLO)和光学相干断层扫描(OCT)进行多模态立体视网膜成像

2018-08-21 cuiguizhong MedSci原创

波士顿大学医学院医学系的Song W等近日在J Vis Exp杂志上发表了一篇文章,介绍一种新的技术方法,将新型的倾斜扫描激光检查眼睛的电镜(oSLO)技术与光学相干断层扫描(OCT)进行结合,可获得3D立体荧光视网膜成像。

波士顿大学医学院医学系的Song W等近日在J Vis Exp杂志上发表了一篇文章,介绍一种新的技术方法,将新型的倾斜扫描激光检查眼睛的电镜(oSLO)技术与光学相干断层扫描(OCT)进行结合,可获得3D立体荧光视网膜成像。

虽然荧光成像技术广泛用于眼科,但大视场(FOV)三维(3D)荧光视网膜图像仍然是视网膜成像模式的一大挑战。因为它们需要进行z轴堆叠编译数据集。较新的光学相干断层扫描(OCT)和OCT血管造影(OCTA)系统克服了这些限制,可以提供三维(3D)解剖结构和血管结构的图像,但OCT的无染料特征不能显示血管功能出现障碍的地方。

这篇文章描述了一种新型的倾斜扫描激光检查眼睛的电镜(oSLO)技术,该技术提供3D立体荧光视网膜成像。成像系统的设置通过滑块产生倾斜扫描,并以最小角度对齐,最终成像系统检测荧光横截面图像。

该系统使用激光扫描的方法,因此可以很容易地结合OCT技术。OCT技术可作为补充,完善体积结构成像模态。在这篇文章中,作者展示了对大鼠视网膜的体内成像。通过静脉内注射荧光素溶液以产生立体荧光血管造影(vFA)。

原文出处:

Song, W., L. Zhou, and J. Yi, Multimodal Volumetric Retinal Imaging by Oblique Scanning Laser Ophthalmoscopy (oSLO) and Optical Coherence Tomography (OCT). J Vis Exp, 2018(138).

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    2019-01-10 habb
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    2018-08-23 zutt
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    2018-08-23 oliver169
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    2018-08-22 哈哈869

    学习了

    0