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CLIN CHEM：多重检测胰腺导管腺癌患者游离DNA 中KRAS突变的预后意义

2018-04-04 MedSci MedSci原创

胰腺导管腺癌患者
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游离DNA（cfDNA）可以作为预测临床结果的潜在生物标志物，但其在胰腺导管腺癌（PDAC）中的价值尚未得到充分评估。本研究的目的是评估PDAC患者游离DNA 中多重KRAS突变定量分析的临床适用性。 共有106名PDAC患者参加了这项前瞻性研究。使用液滴数字PCR试剂盒（Bio-Rad公司）检测血浆样品中的KRAS突变的浓度和片段。 结果显示，在96.1％的组织样本中可检测到KRAS

游离DNA（cfDNA）可以作为预测临床结果的潜在生物标志物，但其在胰腺导管腺癌（PDAC）中的价值尚未得到充分评估。本研究的目的是评估PDAC患者游离DNA 中多重KRAS突变定量分析的临床适用性。 共有106名PDAC患者参加了这项前瞻性研究。使用液滴数字PCR试剂盒（Bio-Rad公司）检测血浆样品中的KRAS突变的浓度和片段。 结果显示，在96.1％的组织样本中可检测到KRAS突变。80例（80.5％）cfDNA存在KRAS突变，中位KRAS突变浓度为0.165份/μL，中位数片段丰度为0.415％。多变量分析表明，KRAS突变浓度[风险比（HR），2.08; 95％CI，1.20-3.63]和KRAS片段（HR，1.73; 95％CI，1.02-2.95）是无进展生存的重要因素。KRAS突变浓度（HR，1.97; 95％CI，1.05-3.67）对总体生存有预后意义。亚组分析显示KRAS突变浓度和片段丰度显著影响可切除PDAC的无进展生存期（P = 0.016）。此外，当与癌症生物标志物CA19-9组合时，cfDNA中的KRAS突变浓度显示出预测总体存活的附加益处。 本研究表

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    2018-04-06 ms5906905816781247

    #KRAS突变#

    0

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    2018-04-06 yinhl1978

    #KRAS#

    0

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    2018-04-06 haouestc

    #预后意义#

    0

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    2018-04-06 LSJ116

    #游离DNA#

    0

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    2018-04-06 bioon7

    #RAS突变#

    0

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