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Stem Cell Res：缺氧及FGF-2的同时应用可增强人骨髓基质细胞的增殖

2018-03-15 MedSci MedSci原创

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本研究旨在确定缺氧或常氧条件下有或无成纤维细胞生长因子-2（FGF-2）处理的培养基中扩增的人骨髓基质细胞（hBMSCs）的细胞特征和行为。将从四组中分离并培养的hBMSCs进行细胞增殖/迁移，集落形成单位，细胞表面表征，体外分化，体内移植和基因表达的评估。使用特定的环境因子（缺氧）和加入FGF-2培养hBMSCs增加了细胞增殖速率，同时增强了再生潜能，调节了多能性相关过程（增强的软骨形成相关过程

本研究旨在确定缺氧或常氧条件下有或无成纤维细胞生长因子-2（FGF-2）处理的培养基中扩增的人骨髓基质细胞（hBMSCs）的细胞特征和行为。

将从四组中分离并培养的hBMSCs进行细胞增殖/迁移，集落形成单位，细胞表面表征，体外分化，体内移植和基因表达的评估。使用特定的环境因子（缺氧）和加入FGF-2培养hBMSCs增加了细胞增殖速率，同时增强了再生潜能，调节了多能性相关过程（增强的软骨形成相关过程/骨生成，但减少了脂肪生成），以及增加细胞迁移和胶原蛋白形成。实验样品中的基因表达水平显示，缺氧条件下缺氧诱导因子-1途径和糖酵解的活化，而不受FGF-2添加的影响。

综上所述，该研究结果表明，由于细胞增殖和再生潜能的增强，低氧和FGF-2的同时应用可以为培养用于与骨组织工程相关的临床应用的hBMSCs提供有利条件。

原始出处：

Lee JS, Kim SK, et al., Enhancing proliferation and optimizing the culture condition for human bone marrow stromal cells using hypoxia and fibroblast growth factor-2. Stem Cell Res. 2018 Jan 9;28:87-95. doi: 10.1016/j.scr.2018.01.010. [Epub ahead of print]

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    2018-05-07 zhaozhouchifen

    #Cell#

    0

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    2018-04-12 维他命

    #CEL#

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    2018-06-14 ms7899726347904398

    #基质#

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encryptionId=82b416e79f5, topicName=stem cell)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=hb2008ye, createdTime=Mon Jun 25 16:29:00 CST 2018, time=2018-06-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2044864, encodeId=4702204486492, content=<a href='/topic/show?id=efbee385a6' target=_blank style='color:#2F92EE;'>#FGF-2#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=7385, encryptionId=efbee385a6, topicName=FGF-2)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqPdWFricgmt6Dde2ptNxJzD6Y9c4cxpXdTo4pNlVPARAnFdKu6nWIPele9icxApTaFgibq0uVlgRiceQ/132, createdBy=4b8c2500026, createdName=ms8435843352634314, createdTime=Wed Sep 05 17:29:00 CST 2018, time=2018-09-05, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1482106, 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CST 2018, time=2018-03-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296530, encodeId=3eaa2965307a, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJpBl6xwMmnsKibwxcAIltOH3nnCEQbNE6TDbYtCKe8hYI0oJrbduNqnJraM1PyyjPKU1suAzicYPaA/132, createdBy=eeaf2031798, createdName=海之蓝, createdTime=Thu Mar 15 17:37:18 CST 2018, time=2018-03-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296529, encodeId=1b8229652994, content=缺氧条件下缺氧诱导因子-1途径和糖酵解的活化.而不受FGF-2添加的影响., beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJUlbw1Mf1vppicGcaWPx1YUOgLgKccao77Rykbx3jiazqzwcyXMdzRIZkhFyBPqknibeuOGhXI1VzVg/0, createdBy=7f162154630, createdName=沙漠浪人, createdTime=Thu Mar 15 17:35:16 CST 2018, time=2018-03-15, status=1, ipAttribution=)]
    2018-06-25 hb2008ye

    #stem cell#

    0

  5. 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createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJUlbw1Mf1vppicGcaWPx1YUOgLgKccao77Rykbx3jiazqzwcyXMdzRIZkhFyBPqknibeuOGhXI1VzVg/0, createdBy=7f162154630, createdName=沙漠浪人, createdTime=Thu Mar 15 17:35:16 CST 2018, time=2018-03-15, status=1, ipAttribution=)]
    2018-09-05 ms8435843352634314

    #FGF-2#

    0

  6. 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CST 2018, time=2018-03-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296530, encodeId=3eaa2965307a, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJpBl6xwMmnsKibwxcAIltOH3nnCEQbNE6TDbYtCKe8hYI0oJrbduNqnJraM1PyyjPKU1suAzicYPaA/132, createdBy=eeaf2031798, createdName=海之蓝, createdTime=Thu Mar 15 17:37:18 CST 2018, time=2018-03-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296529, encodeId=1b8229652994, content=缺氧条件下缺氧诱导因子-1途径和糖酵解的活化.而不受FGF-2添加的影响., beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJUlbw1Mf1vppicGcaWPx1YUOgLgKccao77Rykbx3jiazqzwcyXMdzRIZkhFyBPqknibeuOGhXI1VzVg/0, createdBy=7f162154630, createdName=沙漠浪人, createdTime=Thu Mar 15 17:35:16 CST 2018, time=2018-03-15, status=1, ipAttribution=)]
    2018-03-17 12498bb3m92(暂无昵称)

    #STEM#

    0

  7. 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encodeId=88e51482106eb, content=<a href='/topic/show?id=8cc416e78f3' target=_blank style='color:#2F92EE;'>#STEM#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16778, encryptionId=8cc416e78f3, topicName=STEM)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d1067833268, createdName=12498bb3m92(暂无昵称), createdTime=Sat Mar 17 08:29:00 CST 2018, time=2018-03-17, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296853, encodeId=1fa129685325, content=学习一下谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zSn0GqHrsfLGSUSR8zwnWWLT4FMl5XH8S58HuZslVkLsAPbXDXaliagK7TOxQfmngKibG1yrDonepCLDkcxGk8hS/0, createdBy=b8282062892, createdName=changjiu, createdTime=Fri Mar 16 18:30:35 CST 2018, time=2018-03-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296530, encodeId=3eaa2965307a, content=学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=43, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJpBl6xwMmnsKibwxcAIltOH3nnCEQbNE6TDbYtCKe8hYI0oJrbduNqnJraM1PyyjPKU1suAzicYPaA/132, createdBy=eeaf2031798, createdName=海之蓝, createdTime=Thu Mar 15 17:37:18 CST 2018, time=2018-03-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=296529, encodeId=1b8229652994, content=缺氧条件下缺氧诱导因子-1途径和糖酵解的活化.而不受FGF-2添加的影响., beContent=null, objectType=article, channel=null, level=null, likeNumber=42, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJUlbw1Mf1vppicGcaWPx1YUOgLgKccao77Rykbx3jiazqzwcyXMdzRIZkhFyBPqknibeuOGhXI1VzVg/0, createdBy=7f162154630, createdName=沙漠浪人, createdTime=Thu Mar 15 17:35:16 CST 2018, time=2018-03-15, status=1, ipAttribution=)]
    2018-03-16 changjiu

    学习一下谢谢

    0

  8. 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createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTJUlbw1Mf1vppicGcaWPx1YUOgLgKccao77Rykbx3jiazqzwcyXMdzRIZkhFyBPqknibeuOGhXI1VzVg/0, createdBy=7f162154630, createdName=沙漠浪人, createdTime=Thu Mar 15 17:35:16 CST 2018, time=2018-03-15, status=1, ipAttribution=)]
    2018-03-15 海之蓝

    学习

    0

  9. 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    2018-03-15 沙漠浪人

    缺氧条件下缺氧诱导因子-1途径和糖酵解的活化.而不受FGF-2添加的影响.

    0