J Physiol:HCN 4起搏通道减弱副交感神经反应,稳定窦房结自发放电

2018-03-04 路凯 “罂粟花”微信号

HCN4通道可以减弱SAN的迷走神经反应,从而稳定了SAN的自发放电。

背景与目的

心率由交感神经和副交感神经系统动态控制,这些系统调节窦房结(SAN)。HCN 4起搏器通道是先天性病态窦房结综合征的著名致病分子.虽然HCN4通道被cAMP激活,但HCN 4基因缺失突变患者仍保留SAN的交感神经反应。

方  法

为了阐明HCN4通道在SAN自主神经调节中的作用,我们研制了一种新型的功能获得性突变小鼠,利用四环素反式激活剂和四环素应答元件,HCN4通道的表达水平可可逆地从野生型小鼠的零变为3倍。我们的遥感心电图记录自由活动的清醒小鼠的心率变异性并分析。我们还评估了SAN对颈迷走神经刺激(CVNS)的反应。

结  果

HCN4的条件性过表达并不能诱发心动过速,但可降低心率变异性。HCN4过表达还可减轻由CVN仅在β-肾上腺素能刺激时引起的心动过缓。相反,HCN4的敲除导致窦性心律失常,并增强副交感神经反应;由CVNS诱导的完全性窦性停顿。在体外,我们比较了乙酰胆碱对单个起搏器细胞自发动作电位的影响,发现在β-肾上腺素能刺激和没有β-肾上腺素能刺激的情况下,HCN 4基因的表达都会引起类似的表型变化。

结  论

我们的研究表明,HCN4通道可以减弱SAN的迷走神经反应,从而稳定了SAN的自发放电。

原始出处:

Kozasa Y, Nakashima N, Ito M,et.al. HCN4 pacemaker channels attenuate the parasympathetic response and stabilize the spontaneous firing of the sinoatrial node. J Physiol. 2018 Jan 9. doi: 10.1113/JP275303.

版权声明:
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料,版权均属于梅斯医学所有。非经授权,任何媒体、网站或个人不得转载,授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章,或“梅斯号”自媒体发布的文章,仅系出于传递更多信息之目的,本站仅负责审核内容合规,其内容不代表本站立场,本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。
在此留言
评论区 (3)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1788839, encodeId=b6701e88839dd, content=<a href='/topic/show?id=866d1421e4c' target=_blank style='color:#2F92EE;'>#Physio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14217, encryptionId=866d1421e4c, topicName=Physio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c689267, createdName=cmj8wellington, createdTime=Thu May 10 15:26:00 CST 2018, time=2018-05-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292938, encodeId=39bd292938f2, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/fhZdOu27e88Voqh19n8eF8cLplo8L4nicKtH0WnE3KFELXns5XDu8rsiceK2HHDZc2SEStia5VV3Pia2QLMVqlWbHw/0, createdBy=6e012139475, createdName=神功盖世, createdTime=Sun Mar 04 13:34:19 CST 2018, time=2018-03-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292927, encodeId=7fd229292e4b, content=谢谢老师的讲解, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zMoyicyo6ia9f4IuLQAwZoxD6Hx4ibd5CMcOCYhStY6oDibbKK6O2X8iaicldO5ib8j1iapOIobIKCGiczU2A/0, createdBy=56251941490, createdName=虈亣靌, createdTime=Sun Mar 04 13:07:54 CST 2018, time=2018-03-04, status=1, ipAttribution=)]
  2. [GetPortalCommentsPageByObjectIdResponse(id=1788839, encodeId=b6701e88839dd, content=<a href='/topic/show?id=866d1421e4c' target=_blank style='color:#2F92EE;'>#Physio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14217, encryptionId=866d1421e4c, topicName=Physio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c689267, createdName=cmj8wellington, createdTime=Thu May 10 15:26:00 CST 2018, time=2018-05-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292938, encodeId=39bd292938f2, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/fhZdOu27e88Voqh19n8eF8cLplo8L4nicKtH0WnE3KFELXns5XDu8rsiceK2HHDZc2SEStia5VV3Pia2QLMVqlWbHw/0, createdBy=6e012139475, createdName=神功盖世, createdTime=Sun Mar 04 13:34:19 CST 2018, time=2018-03-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292927, encodeId=7fd229292e4b, content=谢谢老师的讲解, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zMoyicyo6ia9f4IuLQAwZoxD6Hx4ibd5CMcOCYhStY6oDibbKK6O2X8iaicldO5ib8j1iapOIobIKCGiczU2A/0, createdBy=56251941490, createdName=虈亣靌, createdTime=Sun Mar 04 13:07:54 CST 2018, time=2018-03-04, status=1, ipAttribution=)]
    2018-03-04 神功盖世

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1788839, encodeId=b6701e88839dd, content=<a href='/topic/show?id=866d1421e4c' target=_blank style='color:#2F92EE;'>#Physio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14217, encryptionId=866d1421e4c, topicName=Physio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=c689267, createdName=cmj8wellington, createdTime=Thu May 10 15:26:00 CST 2018, time=2018-05-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292938, encodeId=39bd292938f2, content=阅, beContent=null, objectType=article, channel=null, level=null, likeNumber=55, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/fhZdOu27e88Voqh19n8eF8cLplo8L4nicKtH0WnE3KFELXns5XDu8rsiceK2HHDZc2SEStia5VV3Pia2QLMVqlWbHw/0, createdBy=6e012139475, createdName=神功盖世, createdTime=Sun Mar 04 13:34:19 CST 2018, time=2018-03-04, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=292927, encodeId=7fd229292e4b, content=谢谢老师的讲解, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3zMoyicyo6ia9f4IuLQAwZoxD6Hx4ibd5CMcOCYhStY6oDibbKK6O2X8iaicldO5ib8j1iapOIobIKCGiczU2A/0, createdBy=56251941490, createdName=虈亣靌, createdTime=Sun Mar 04 13:07:54 CST 2018, time=2018-03-04, status=1, ipAttribution=)]
    2018-03-04 虈亣靌

    谢谢老师的讲解

    0