Arch Oral Biol:研究发现Coronarin D可用于治疗牙周炎

2019-09-03 不详 网络

牙周炎是最常见的口腔疾病,可以破坏牙周支持组织,导致牙齿脱落。抗炎物质可有效改善牙周炎。本研究旨在探讨Coronarin D能否解决牙周炎的发生,改善人牙周膜干细胞(hPDLSCs)的增殖和成骨分化。在用TNF-α或Coronarin D处理后,使用CCK-8测定来检测hPDLSC的存活力。碱性磷酸酶(ALP)活性试剂盒和茜素红染色用于确定hPDLSC的ALP活性和矿化成骨能力。通过蛋白质印迹检测

牙周炎是最常见的口腔疾病,可以破坏牙周支持组织,导致牙齿脱落。抗炎物质可有效改善牙周炎。本研究旨在探讨Coronarin D能否解决牙周炎的发生,改善人牙周膜干细胞(hPDLSCs)的增殖和成骨分化。

在用TNF-α或Coronarin D处理后,使用CCK-8测定来检测hPDLSC的存活力。碱性磷酸酶(ALP)活性试剂盒和茜素红染色用于确定hPDLSC的ALP活性和矿化成骨能力。通过蛋白质印迹检测细胞周期蛋白(CDK2,cyclinE1,P27),分化相关蛋白(Oct4,Sox2,Runx2)和抗炎信号蛋白(TNF-α,NF / KBp50)。

结果表明,Coronarin D可以改善TNF-α诱导的hPDLSCs的活力,增殖,分化和成矿作用。并且,TNF-α诱导的hPDLSCs后,细胞周期蛋白(CDK2,cyclinE1,P27),分化相关蛋白(Oct4,Sox2,Runx2)和抗炎信号蛋白(TNF-α,NF/KBp50)的表达发生了变化。

结论,所提供的数据表明,Coronarin D可以改善TNF-α诱导的人牙周膜干细胞的增殖和成骨分化。

原始出处:

Jiang C, Wang Q, et al., Coronarin D affects TNF-α induced proliferation and osteogenic differentiation of human periodontal ligament stem cells. Arch Oral Biol. 2019 Aug 12;108:104519. doi: 10.1016/j.archoralbio.2019.104519.

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    2020-06-28 小几洁
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    2020-05-31 sunylz
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