Arch Oral Biol:百里香提取物可有效发挥抗菌抗炎作用

2018-04-15 MedSci MedSci原创

本研究评估了百里香提取物的生物学效应,如对浮游生物培养和单及多微生物生物膜的抗菌活性、细胞毒性、抗炎活性和遗传毒性。 白色念珠菌、金黄色葡萄球菌、粪肠球菌、变形链球菌和铜绿假单胞菌的单菌生物膜以及由白色念珠菌与每种细菌组成的多种微生物生物膜形成48小时并暴露于植物提取物5分钟。将小鼠巨噬细胞(RAW 264.7)、人牙龈成纤维细胞(FMM-1)、人乳腺癌细胞(MCF-7)和宫颈癌细胞(HeLa)暴

本研究评估了百里香提取物的生物学效应,如对浮游生物培养和单及多微生物生物膜的抗菌活性、细胞毒性、抗炎活性和遗传毒性。

白色念珠菌、金黄色葡萄球菌、粪肠球菌、变形链球菌和铜绿假单胞菌的单菌生物膜以及由白色念珠菌与每种细菌组成的多种微生物生物膜形成48小时并暴露于植物提取物5分钟。将小鼠巨噬细胞(RAW 264.7)、人牙龈成纤维细胞(FMM-1)、人乳腺癌细胞(MCF-7)和宫颈癌细胞(HeLa)暴露于植物提取物中5分钟,采用MTT法、中性红(NR)和结晶紫(CV)分析检测细胞活力。暴露于植物提取物24小时后,通过ELISA定量分析RAW 264.7产生的白细胞介素-1β(IL-1β)和肿瘤坏死因子α(TNF-α)。通过1000个细胞中的微核形成(MN)评估植物提取物的基因毒性。结果通过T检验或ANOVA和Tukey检验分析(P≤0.05)。

结果显示,所有生物膜显示CFU/mL(菌落形成单位/毫升)明显降低。所有细胞系的细胞活力高于50%。还观察到对IL-1β和TNF-α合成的抗炎作用。在所有细胞中,MN与对照组相似或更低。

综上所述,该研究结果表明,百里香提取物对所有生物膜有效,促进高细胞活力,具有抗炎作用并且没有遗传毒性。

原始出处:

Oliveira JR, de Jesus Viegas D, et al., Thymus vulgaris L. extract has antimicrobial and anti-inflammatory effects in the absence of cytotoxicity and genotoxicity. Arch Oral Biol. 2017 Oct;82:271-279. doi: 10.1016/j.archoralbio.2017.06.031. Epub 2017 Jun 27.

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    2018-12-02 小几洁
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    2018-06-28 sunylz
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