CCLM：对SSA/Ro52抗原p200抗原表位的自身抗体检测情况如何？

2018-06-01 MedSci MedSci原创

抗p200抗体由于其与胎儿自身免疫性先天性心脏阻滞(CHB)相关的发现而受到越来越多的关注。本研究比较并评估了目前用于该检测的两种检测方法的性能。研究对象为1616名抗ssa /Ro52抗体阳性的孕妇。另外抗ssa /Ro52抗体阴性的50名妇女为对照。抗p200抗体的分析采用两种自制的ELISA法:一种是生物素化抗原，另一种是自由抗原。结果: 与p200-生物素试验相比，无

抗p200抗体由于其与胎儿自身免疫性先天性心脏阻滞(CHB)相关的发现而受到越来越多的关注。本研究比较并评估了目前用于该检测的两种检测方法的性能。研究对象为1616名抗ssa /Ro52抗体阳性的孕妇。另外抗ssa /Ro52抗体阴性的50名妇女为对照。抗p200抗体的分析采用两种自制的ELISA法:一种是生物素化抗原，另一种是自由抗原。结果: 与p200-生物素试验相比，无p200的特异性明显更高(p=0.023)。两种方法在曲线下的面积都比较大，精度较高。当采用不含p200的检测方法分析携带CHB胎儿的孕妇的血清时，抗p200抗体的流行程度较为显著(p=0.007)。Cohen’s κ 和 Spearman’s ρρ系数显示出良好的一致性(0.71)，相关性较高(0.93)。与基于生物素的方法相比，无p200方法在检测抗ssa /Ro52抗体阳性的妇女的p200抗体时更为明确。此外，只有无p200的方法在胎儿CHB患者中发现了p200抗体原始出处： Mattia, Elena, Detection of autoantibodies to the p200-epitope

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2020-11-27 zhoujin4150

太贵

59 0
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2018-06-03 zhaojie88

#体检#

38 0
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2018-06-03 tongyongming

#抗体检测#

26 0
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2018-06-03 xiaoyeshuang

#抗原#

40 0
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2018-06-02 1ddf0692m34(暂无匿称)

学习了.长知识

62 0
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2018-06-01 1ddf0692m34(暂无匿称)

学习了.长知识

61 0
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2018-06-01 139****5926

好好文章学习了

74 0

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