Eur Cell Mater:3D打印多孔支架有利于骨缺损的修复

2019-10-13 不详 网络

互连的孔隙度对于再生支架的设计至关重要,因为它允许细胞迁移,血管化以及营养物和调节分子在支架内部扩散。3D打印是实现此目标的一种有前途的策略,因为它可以控制支架的孔径,孔隙率和互连性。因此,本研究旨在整合独特的生物加工策略,以开发出一种多尺度的多孔支架,该支架不仅在植入时具有机械功能,而且还促进了快速血管形成,并为干细胞提供了适当的线索以使其分化为成骨细胞。为此,将聚己内酯(PCL)与脱细胞的骨细

互连的孔隙度对于再生支架的设计至关重要,因为它允许细胞迁移,血管化以及营养物和调节分子在支架内部扩散。3D打印是实现此目标的一种有前途的策略,因为它可以控制支架的孔径,孔隙率和互连性。因此,本研究旨在整合独特的生物加工策略,以开发出一种多尺度的多孔支架,该支架不仅在植入时具有机械功能,而且还促进了快速血管形成,并为干细胞提供了适当的线索以使其分化为成骨细胞。

为此,将聚己内酯(PCL)与脱细胞的骨细胞外基质(ECM)功能化,以生产用于3D打印的骨诱导丝。向PCL中添加骨ECM不仅增加了所得支架的机械性能,而且还增加了细胞附着并增强了间充质干细胞(MSC)的成骨作用。在体内,支架的孔径决定了血管化的水平,较大的丝间距支持更快的血管向内生长和更多的新骨形成。通过在这些3D打印的支架中冻干溶解的骨ECM,可以引入具有微孔孔隙度的基质网络,从而进一步增强体外细胞附着力并增加血管浸润和体内新骨形成的总体水平。

总而言之,开发了一种“现成的”多尺度骨ECM衍生支架,该支架机械稳定,一旦植入体内,将驱动血管形成,并最终导致骨再生。

原始出处:

Freeman FE, Browe DC, et al., Biofabrication of multiscale bone extracellular matrix scaffolds for bone tissue engineering. Eur Cell Mater. 2019 Oct 11;38:168-187. doi: 10.22203/eCM.v038a12.

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    2020-09-22 维他命
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    2020-04-26 医者仁心
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    2019-10-15 lfcmxl