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Exp Ther Med：YAP的下调抑制Eca-109细胞的增殖并诱导细胞凋亡

2018-02-10 MedSci MedSci原创

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研究报道，在食管鳞状细胞癌（ESCC）中，Y相关蛋白（YAP）基因过表达，然而，YAP在ESCCs中的确切作用仍然不清楚。本研究旨在探讨YAP对ESCC的抑制作用。为了研究YAP在ESCC细胞中的确切作用，使用YAP-小干扰RNA建立稳定的YAP低表达ESCC细胞系。MTT法检测细胞增殖能力，流式细胞术检测细胞凋亡和细胞周期分布。另外，分别应用逆转录-定量聚合酶链式反应和蛋白质印迹分析进行mRNA

研究报道，在食管鳞状细胞癌（ESCC）中，Y相关蛋白（YAP）基因过表达，然而，YAP在ESCCs中的确切作用仍然不清楚。本研究旨在探讨YAP对ESCC的抑制作用。

为了研究YAP在ESCC细胞中的确切作用，使用YAP-小干扰RNA建立稳定的YAP低表达ESCC细胞系。MTT法检测细胞增殖能力，流式细胞术检测细胞凋亡和细胞周期分布。另外，分别应用逆转录-定量聚合酶链式反应和蛋白质印迹分析进行mRNA和蛋白质水平检测。

结果显示，YAP基因的抑制作用明显抑制ECA-109细胞的增殖，诱导细胞凋亡，而这种抑制作用对细胞周期无明显影响。此外，本研究确定了细胞凋亡相关蛋白的表达水平，结果还表明YAP基因抑制ECA-109细胞中，B细胞淋巴瘤2（Bcl-2）/Bcl-2相关的X蛋白比率和磷酸化细胞外信号调节激酶的表达显著降低，而p53和caspase 3的表达明显增加。

综上所述，目前的研究表明，抑制YAP基因可抑制ECA-109细胞的增殖并诱导细胞凋亡，表明YAP基因或是ESCC的一种癌基因。

原始出处：

Cui M, Li Z. Downregulation of YAP inhibits proliferation and induces apoptosis in Eca-109 cells. Exp Ther Med. 2018 Jan;15(1):1048-1052. doi: 10.3892/etm.2017.5492. Epub 2017 Nov 10.

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    2018-06-02 gujh

    #YAP#

    0

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    2018-02-12 ms8538335253054589

    #细胞凋亡#

    0

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    2018-02-12 ms3994565386320060

    #Med#

    0

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    2018-02-10 虈亣靌

    学习谢谢分享

    0

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    2018-02-10 changjiu

    学习一下谢谢

    0

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    2018-02-10 131****1460

    学习了受益匪浅

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