纳米复合支架中引入BG纳米纤维可更好的促进成骨

2018-01-20 MedSci MedSci原创

采用静电纺丝法制备了SiO2-CaO-P2O5生物活性玻璃(BG)纳米纤维,聚-L-乳酸(PLLA)纳米纤维和纳米复合材料支架。通过XRD、FTIR和SEM技术对所制备的纳米纤维和支架进行表征,纳米纤维的平均直径约为500nm,完全的纤维性支架具有多孔结构和相互连接的孔。采用MTT法、碱性磷酸酶活性、钙沉积量、茜素红染色和RT-PCR等多种生物学方法研究了培养的人骨髓间充质干细胞在纳米纤维和生物活

采用静电纺丝法制备了SiO2-CaO-P2O5生物活性玻璃(BG)纳米纤维,聚-L-乳酸(PLLA)纳米纤维和纳米复合材料支架。

通过XRD、FTIR和SEM技术对所制备的纳米纤维和支架进行表征,纳米纤维的平均直径约为500nm,完全的纤维性支架具有多孔结构和相互连接的孔。采用MTT法、碱性磷酸酶活性、钙沉积量、茜素红染色和RT-PCR等多种生物学方法研究了培养的人骨髓间充质干细胞在纳米纤维和生物活性玻璃聚乳酸(BG-PLLA)纳米复合支架材料上的生长、活性和增殖情况。

结果显示,在纳米复合支架中引入BG纳米纤维可优化支架的生物学行为。此外,与组分相比,支架的三维和纤维多孔结构进一步有助于其细胞行为的改善。

原始出处:


Shamsi M, Karimi M, et al., In vitro proliferation and differentiation of human bone marrow mesenchymal stem cells into osteoblasts on nanocomposite scaffolds based on bioactive glass (64SiO2-31CaO-5P2O5)-poly-l-lactic acid nanofibers fabricated by electrospinning method. Mater Sci Eng C Mater Biol Appl. 2017 Sep 1;78:114-123. doi: 10.1016/j.msec.2017.02.165. Epub 2017 Mar 1.

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  2. [GetPortalCommentsPageByObjectIdResponse(id=1635672, encodeId=4dff16356e236, content=<a href='/topic/show?id=1059e743357' target=_blank style='color:#2F92EE;'>#纳米纤维#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=20, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77433, encryptionId=1059e743357, topicName=纳米纤维)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=64fe22519052, createdName=1249883fm19暂无昵称, createdTime=Sat Apr 21 22:53:00 CST 2018, time=2018-04-21, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=281404, encodeId=4bdc281404c9, content=非常好的文章.学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo7LIiaRZTzJ3SEfiauCzTMPW7YvPPYLNJBXG9oh6Al1icq2VQQkIHWxqXehcicTS62YKJVBhxeth3wggw/0, createdBy=7ce01621306, createdName=天涯183, createdTime=Tue Jan 23 23:50:04 CST 2018, time=2018-01-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1351651, encodeId=563d13516512f, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=16, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Mon Jan 22 06:53:00 CST 2018, time=2018-01-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=280422, encodeId=0137280422a1, content=学习一下, beContent=null, objectType=article, channel=null, level=null, likeNumber=47, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/NUyjXTCJjo6KcicAVC1MwuOgo3iaicb1Imgk1yIfnvr6oXzibcAFB6puAyNJXbzWhNuLqaFYBVRWjh7rghkvFLPxMAfD5ib3nXwVD/0, createdBy=bed21972958, createdName=wxl882001, createdTime=Sat Jan 20 18:23:41 CST 2018, time=2018-01-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=280412, encodeId=0df028041250, content=^_^^_^^_^^_^^_^^_^, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/vi_32/Swp2ehfHpiciczeTntUyQOlIGMzQ705ic67sdMTVMbwTv7KbmlUvxrGvRSvQZjPkz7ib5tEDfIaORgczPtmHDpwojg/0, createdBy=c54b2163125, createdName=wqkm, createdTime=Sat Jan 20 17:24:46 CST 2018, time=2018-01-20, status=1, ipAttribution=)]
    2018-01-23 天涯183

    非常好的文章.学习了

    0

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    2018-01-22 zhaojie88
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    2018-01-20 wxl882001

    学习一下

    0

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    2018-01-20 wqkm

    ^_^^_^^_^^_^^_^^_^

    0