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Onco Targets Ther：研究揭示NEAT1在卵巢癌中的表达及功能

2017-11-04 MedSci MedSci原创

NEAT1卵巢癌miR-34a-5p
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研究已经证明富含核富集的转录物1（NEAT1）在许多癌症中起着肿瘤抑制剂的作用。然而，NEAT1在卵巢癌（OC）发展中的作用仍有待进一步明确。因此，本研究的目的是探讨NEAT1在OC中的表达和功能。通过定量实时聚合酶链式反应测定OC细胞系中NEAT1的表达水平。MTT法检测caspase-3活性，流式细胞术检测NEAT1、miR-34a-5p或B细胞淋巴瘤-2（BCL2）对OC细胞增殖和凋亡的影响

研究已经证明富含核富集的转录物1（NEAT1）在许多癌症中起着肿瘤抑制剂的作用。然而，NEAT1在卵巢癌（OC）发展中的作用仍有待进一步明确。因此，本研究的目的是探讨NEAT1在OC中的表达和功能。

通过定量实时聚合酶链式反应测定OC细胞系中NEAT1的表达水平。MTT法检测caspase-3活性，流式细胞术检测NEAT1、miR-34a-5p或B细胞淋巴瘤-2（BCL2）对OC细胞增殖和凋亡的影响。荧光素酶报告基因测定法用于证实OC细胞中NEAT1、BCL2和miR-34a-5p的相互作用。

结果显示，NEAT1在OC细胞系中明显上调。NEAT1过度表达通过增加S期细胞比例并抑制OC细胞凋亡来促进细胞的增殖，而敲减NEAT1则会产生相反的效果。此外，NEAT1可与miR-34a-5p直接相互作用，并通过负调控miR-34a-5p在OC中发挥其致癌作用。此外，miR-34a-5p可直接靶向BCL2并抑制其表达。miR-34a-5p过表达抑制OC细胞增殖并通过靶向BCL2触发细胞凋亡。此外，NEAT1敲低可抑制BCL2的表达，而抗miR-34a-5p则会明显降低si-NEAT1对BCL2表达的抑制作用。

总之，该研究发现，NEAT1通过miR-34a-5p/BCL2调节OC细胞的增殖和凋亡，为OC患者的治疗提供了一种潜在的方法。

原始出处：


Nan Ding, Haiying Wu, Tao Tao, Erxuan Peng. NEAT1 regulates cell proliferation and apoptosis of ovarian cancer by miR-34a-5p/BCL2. Onco Targets Ther. 2017; 10: 4905–4915. Published online 2017 Oct 6. doi: 10.2147/OTT.S142446

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评论区 (3)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=2030222, encodeId=9209203022281, content=<a href='/topic/show?id=795d1260963' target=_blank style='color:#2F92EE;'>#NEAT1#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12609, encryptionId=795d1260963, topicName=NEAT1)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3fc52500170, createdName=12498cbcm00(暂无昵称), createdTime=Sat Sep 01 09:08:00 CST 2018, time=2018-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1795484, encodeId=11b71e9548492, content=<a href='/topic/show?id=248e1e1345b' target=_blank style='color:#2F92EE;'>#target#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17134, encryptionId=248e1e1345b, topicName=target)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Sun Nov 12 23:08:00 CST 2017, time=2017-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=258679, encodeId=d2652586e983, content=学习了受益匪浅, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLDicHg2ldsSNmib46PmdzpO7CQEmPjuULp1v45zPWwMYhrnXgvl3OtmUCZSQfgITWn96X9moL94nUGA/0, createdBy=28072041016, createdName=131****1460, createdTime=Sat Nov 04 19:24:13 CST 2017, time=2017-11-04, status=1, ipAttribution=)]
    2018-09-01 12498cbcm00(暂无昵称)

    #NEAT1#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=2030222, encodeId=9209203022281, content=<a href='/topic/show?id=795d1260963' target=_blank style='color:#2F92EE;'>#NEAT1#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12609, encryptionId=795d1260963, topicName=NEAT1)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3fc52500170, createdName=12498cbcm00(暂无昵称), createdTime=Sat Sep 01 09:08:00 CST 2018, time=2018-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1795484, encodeId=11b71e9548492, content=<a href='/topic/show?id=248e1e1345b' target=_blank style='color:#2F92EE;'>#target#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17134, encryptionId=248e1e1345b, topicName=target)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Sun Nov 12 23:08:00 CST 2017, time=2017-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=258679, encodeId=d2652586e983, content=学习了受益匪浅, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLDicHg2ldsSNmib46PmdzpO7CQEmPjuULp1v45zPWwMYhrnXgvl3OtmUCZSQfgITWn96X9moL94nUGA/0, createdBy=28072041016, createdName=131****1460, createdTime=Sat Nov 04 19:24:13 CST 2017, time=2017-11-04, status=1, ipAttribution=)]
    2017-11-12 仁医06

    #target#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=2030222, encodeId=9209203022281, content=<a href='/topic/show?id=795d1260963' target=_blank style='color:#2F92EE;'>#NEAT1#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12609, encryptionId=795d1260963, topicName=NEAT1)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3fc52500170, createdName=12498cbcm00(暂无昵称), createdTime=Sat Sep 01 09:08:00 CST 2018, time=2018-09-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1795484, encodeId=11b71e9548492, content=<a href='/topic/show?id=248e1e1345b' target=_blank style='color:#2F92EE;'>#target#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=17134, encryptionId=248e1e1345b, topicName=target)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=55683, createdName=仁医06, createdTime=Sun Nov 12 23:08:00 CST 2017, time=2017-11-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=258679, encodeId=d2652586e983, content=学习了受益匪浅, beContent=null, objectType=article, channel=null, level=null, likeNumber=63, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLDicHg2ldsSNmib46PmdzpO7CQEmPjuULp1v45zPWwMYhrnXgvl3OtmUCZSQfgITWn96X9moL94nUGA/0, createdBy=28072041016, createdName=131****1460, createdTime=Sat Nov 04 19:24:13 CST 2017, time=2017-11-04, status=1, ipAttribution=)]
    2017-11-04 131****1460

    学习了受益匪浅

    0