Biochem Biophys Res Commun:氧化石墨烯掺杂的nHAC/PLGA支架促进MC3T3-E1细胞的增殖

2018-01-31 MedSci MedSci原创

石墨烯是一种典型的二维(2D)材料,已知其可影响干细胞的多种行为,包括细胞的粘附、扩散、生长和分化。本研究中,研究人员首次报道了四种不同新兴2D材料对人类脂肪间充质干细胞(hADMSCs)的影响。研究人员选择氧化石墨烯(GO)、硫化钼(MoS 2)、硫化钨(WS 2)和氮化硼(BN)作为模型二维材料,并通过滴铸法涂布在细胞培养基材上。在低浓度范围(<5μg/ ml),四种不同的2D材料中的任

石墨烯是一种典型的二维(2D)材料,已知其可影响干细胞的多种行为,包括细胞的粘附、扩散、生长和分化。本研究中,研究人员首次报道了四种不同新兴2D材料对人类脂肪间充质干细胞(hADMSCs)的影响。

研究人员选择氧化石墨烯(GO)、硫化钼(MoS 2)、硫化钨(WS 2)和氮化硼(BN)作为模型二维材料,并通过滴铸法涂布在细胞培养基材上。在低浓度范围(<5μg/ ml),四种不同的2D材料中的任何一种均未观察到急性毒性。有趣的是,当与未处理的(NT)底物相比时,2D材料改性的底物表现出更高的细胞粘附、延伸和增殖。

值得注意的是,在分化的情况下,mRNA表达水平和ORO染色结果显示,与NT和GO修饰的底物相比,MoS2-,WS2-和BN-修饰的底物表现出更好的性能,包括诱导hADMSCs的脂肪生成。相反,发现在所有2D-材料涂覆的基底中,GO-涂布的基底(50μg/mL)诱导成骨的效果最佳。

总之,2D材料可以作为控制干细胞生长和分化的有利来源,这在生物医学研究和治疗中可能是非常有利的。

原始出处:


Suhito IR, Han Y, et al., Effects of two-dimensional materials on human mesenchymal stem cell behaviors. Biochem Biophys Res Commun. 2017 Nov 4;493(1):578-584. doi: 10.1016/j.bbrc.2017.08.149. Epub 2017 Sep 6.

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    2018-05-29 sunylz
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    2018-02-26 liuli5079
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level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLBkyaelI9ia3n9SbuKmBdaY0PqCOzd0nhRUstroXmUmXZ5EBTmibtLEvCCIpyHAqxQXGfZz49ibrwjkg/0, createdBy=39781652829, createdName=雾夜芭蕾, createdTime=Thu Feb 01 07:52:58 CST 2018, time=2018-02-01, status=1, ipAttribution=)]
    2018-12-28 venlin
  5. 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level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLBkyaelI9ia3n9SbuKmBdaY0PqCOzd0nhRUstroXmUmXZ5EBTmibtLEvCCIpyHAqxQXGfZz49ibrwjkg/0, createdBy=39781652829, createdName=雾夜芭蕾, createdTime=Thu Feb 01 07:52:58 CST 2018, time=2018-02-01, status=1, ipAttribution=)]
    2018-02-03 txqjm

    谢谢了.学习

    0

  8. 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createdName=yangjxjc, createdTime=Sat Sep 15 06:03:00 CST 2018, time=2018-09-15, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2087273, encodeId=3602208e273db, content=<a href='/topic/show?id=490750196b' target=_blank style='color:#2F92EE;'>#COMMUN#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=5019, encryptionId=490750196b, topicName=COMMUN)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=7931272, createdName=liuli5079, createdTime=Mon Feb 26 02:03:00 CST 2018, time=2018-02-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1704800, encodeId=632c1e0480015, content=<a href='/topic/show?id=0d28143e392' target=_blank style='color:#2F92EE;'>#PLGA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=14373, encryptionId=0d28143e392, topicName=PLGA)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c02c30837939, createdName=venlin, createdTime=Fri Dec 28 18:03:00 CST 2018, time=2018-12-28, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1706962, encodeId=98b91e06962fb, content=<a href='/topic/show?id=d2773359191' target=_blank style='color:#2F92EE;'>#化石#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=33591, encryptionId=d2773359191, topicName=化石)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f4d531331396, createdName=ms7899726347904398, createdTime=Tue Nov 20 00:03:00 CST 2018, time=2018-11-20, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1707536, encodeId=403b1e07536d1, content=<a href='/topic/show?id=9f7c99e256' target=_blank style='color:#2F92EE;'>#IOP#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=9972, encryptionId=9f7c99e256, topicName=IOP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=6bb031431834, createdName=124987c6m46暂无昵称, createdTime=Fri Feb 16 06:03:00 CST 2018, time=2018-02-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284664, encodeId=94cc284664c9, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Sat Feb 03 07:23:21 CST 2018, time=2018-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284294, encodeId=338d2842949b, content=学习了很有用不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Fri Feb 02 00:17:00 CST 2018, time=2018-02-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284108, encodeId=d47128410882, content=学习了新知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20201006/4db877c2887649beb09cb5effd0b38c7/916f66edd48a4e77b20ebc3e14b6e45c.jpg, createdBy=141e1980133, createdName=Y—xianghai, createdTime=Thu Feb 01 08:19:27 CST 2018, time=2018-02-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284102, encodeId=7ebc284102c6, content=最后再加一遍吡啊, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLBkyaelI9ia3n9SbuKmBdaY0PqCOzd0nhRUstroXmUmXZ5EBTmibtLEvCCIpyHAqxQXGfZz49ibrwjkg/0, createdBy=39781652829, createdName=雾夜芭蕾, createdTime=Thu Feb 01 07:52:58 CST 2018, time=2018-02-01, status=1, ipAttribution=)]
    2018-02-02 飛歌

    学习了很有用不错

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=1917915, encodeId=2cab191e9158d, content=<a href='/topic/show?id=4f26341994' target=_blank style='color:#2F92EE;'>#Bio#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=3419, encryptionId=4f26341994, topicName=Bio)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=12de429, createdName=sunylz, createdTime=Tue May 29 18:03:00 CST 2018, time=2018-05-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1651544, encodeId=e5b0165154493, content=<a href='/topic/show?id=6e78e32573c' target=_blank style='color:#2F92EE;'>#石墨烯#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=32, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=73257, encryptionId=6e78e32573c, topicName=石墨烯)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=8db924213398, 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objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=9972, encryptionId=9f7c99e256, topicName=IOP)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=6bb031431834, createdName=124987c6m46暂无昵称, createdTime=Fri Feb 16 06:03:00 CST 2018, time=2018-02-16, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284664, encodeId=94cc284664c9, content=谢谢了.学习, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=30331616752, createdName=txqjm, createdTime=Sat Feb 03 07:23:21 CST 2018, time=2018-02-03, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284294, encodeId=338d2842949b, content=学习了很有用不错, beContent=null, objectType=article, channel=null, level=null, likeNumber=37, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Fri Feb 02 00:17:00 CST 2018, time=2018-02-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284108, encodeId=d47128410882, content=学习了新知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20201006/4db877c2887649beb09cb5effd0b38c7/916f66edd48a4e77b20ebc3e14b6e45c.jpg, createdBy=141e1980133, createdName=Y—xianghai, createdTime=Thu Feb 01 08:19:27 CST 2018, time=2018-02-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284102, encodeId=7ebc284102c6, content=最后再加一遍吡啊, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLBkyaelI9ia3n9SbuKmBdaY0PqCOzd0nhRUstroXmUmXZ5EBTmibtLEvCCIpyHAqxQXGfZz49ibrwjkg/0, createdBy=39781652829, createdName=雾夜芭蕾, createdTime=Thu Feb 01 07:52:58 CST 2018, time=2018-02-01, status=1, ipAttribution=)]
    2018-02-01 Y—xianghai

    学习了新知识

    0

  10. 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topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/oLAjfB7s1ib3IzoRn621JdKCFUI4XvNELcibocrAVU6aUzEVDSaf3rYH08PUYUiagGqxAEhj8g9WqqNvD1CupIpCrmnR2OrqHZh/0, createdBy=7f181365728, createdName=飛歌, createdTime=Fri Feb 02 00:17:00 CST 2018, time=2018-02-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284108, encodeId=d47128410882, content=学习了新知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=49, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20201006/4db877c2887649beb09cb5effd0b38c7/916f66edd48a4e77b20ebc3e14b6e45c.jpg, createdBy=141e1980133, createdName=Y—xianghai, createdTime=Thu Feb 01 08:19:27 CST 2018, time=2018-02-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=284102, encodeId=7ebc284102c6, content=最后再加一遍吡啊, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/ajNVdqHZLLBkyaelI9ia3n9SbuKmBdaY0PqCOzd0nhRUstroXmUmXZ5EBTmibtLEvCCIpyHAqxQXGfZz49ibrwjkg/0, createdBy=39781652829, createdName=雾夜芭蕾, createdTime=Thu Feb 01 07:52:58 CST 2018, time=2018-02-01, status=1, ipAttribution=)]
    2018-02-01 雾夜芭蕾

    最后再加一遍吡啊

    0