Exp Ther Med:壳聚糖微球体外重组人骨形成蛋白对大鼠异位成骨的影响

2019-04-24 不详 网络

在本研究中,研究人员在大鼠模型中探究了由壳聚糖(CS)微球递送的重组人骨形态发生蛋白-2(rhBMP-2)对异位成骨的影响。制备负载rhBMP-2的CS微球和空白CS微球。将24只雄性Sprague Dawley大鼠分成4组,每组6只:CS/rhBMP-2组,rhBMP-2组,其中直接植入rhBMP-2(rhBMP-2剂量为1mg),CS空白组和对照组。在异位成骨手术后4周进行X射线检查,并在手术

在本研究中,研究人员在大鼠模型中探究了由壳聚糖(CS)微球递送的重组人骨形态发生蛋白-2(rhBMP-2)对异位成骨的影响。

制备负载rhBMP-2的CS微球和空白CS微球。将24只雄性Sprague Dawley大鼠分成4组,每组6只:CS/rhBMP-2组,rhBMP-2组,其中直接植入rhBMP-2(rhBMP-2剂量为1mg),CS空白组和对照组。在异位成骨手术后4周进行X射线检查,并在手术后1,2,3和4周进行微型计算机断层扫描(CT)检查,以确定不同组的异位成骨。此外,研究人员还进行了组织学分析,碱性磷酸酶(ALP)活性和钙含量的测定。

结果显示,手术后4周CS/rhBMP-2组骨样组织的平均直径为1.1±0.3 cm(范围0.8-1.4 cm),明显高于大于rhBMP-2组(0.3±0.1 cm;范围,0.1-0.4 cm)。X射线分析和微CT扫描显示CS/rhBMP-2组中高密度组织面积和放射性核素强度以及三维重建中的骨量最大,其次是rhBMP-2组。与rhBMP-2组相比,CS / rhBMP-2组在手术后3周和4周时所有参数(包括骨矿物质密度,组织矿物质密度,组织矿物质含量和骨体积分数)均显著增高。组织学分析,ALP活性分析和钙含量的测定表明CS/rhBMP-2系统诱导成骨细胞分化的能力最大。

总之,CS/rhBMP-2微球递送系统显著增强了rhBMP-2对异位成骨的诱导和促进作用。本研究增强了CS/rhBMP-2微球输送系统未来应用的基础数据,并更深入地了解BMP-2在骨再生中的作用。

原始出处:

Xia YJ, Wei W, et al., Effect of recombinant human bone morphogenetic protein delivered by chitosan microspheres on ectopic osteogenesis in rats. Exp Ther Med. 2019 May;17(5):3891-3898. doi: 10.3892/etm.2019.7406. Epub 2019 Mar 18.

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    2019-05-16 zwjnj2

    努力学习天天学习认真学习

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    2019-04-26 zhaojie88
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