Nature:你知道生命王国的蛋白质组图谱是怎么样的吗?

2020-06-26 MedSci原创 MedSci原创

该研究结果为整个进化范围内的生物体功能组织提供了一个可以相互比较的视角。

众所周知的是,蛋白质在所有生物领域中发挥了绝大多数的功能,但由于技术原因,其大规模的研究已经落后于基因组的研究。

从第一个基本完整的真核生物蛋白质组的报告以来,质谱方法的进步使人类蛋白质组得以越来越全面的识别和量化。

然而,至今为止,一直很少有跨物种的蛋白质组比较,这与基因组学所倡导的观念形成了鲜明对比。

在这里,研究人员使用一个先进的蛋白质组学工作流程,其中肽分离步骤是由一个微结构和极其可重复的色谱系统进行的,该系统可用来深入研究100种分类学上的不同生物。

研究人员以标准化的方式获得了200万个肽段和34万个严格的蛋白质组分,使科学界已知的具有可靠实验证据的蛋白质数量增加了一倍。这些数据也为基于序列的机器学习提供了一个大规模的案例研究,研究人员还通过实验证实了来自统一细菌的肽段的预测特性。

该研究结果为整个进化范围内的生物体功能组织提供了一个可以相互比较的视角。在所有王国的蛋白质组中,有相当高的部分被用于蛋白质的稳态和折叠,这突出了在所有生命分支中维持蛋白质结构的生物挑战。

同样,普遍较高的蛋白部分也参与了能源供应,尽管这些途径有光合作用,铁硫代谢,以及碳水化合物代谢的区别。然而,一般来说,生物体之间的蛋白质和蛋白质组是非常不同的,人们可以很容易地在www.proteomesoflife.org 网站上对这些数据进行探索和功能比较。

 

原始出处:

Johannes B. Müller et al. The proteome landscape of the kingdoms of life, Nature (2020). DOI:10.1038/s41586-020-2402-x

 

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    2020-10-03 liye789132251
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