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Int J Mol Sci：无异种条件产生的间充质干细胞/细胞外基质复合物团块可修复骨缺损

2019-09-03 不详 网络

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间充质干细胞（MSC）/细胞外基质（ECM）复合物（C-MSC）的三维团块由细胞和自身产生的ECM组成。我们以前证明C-MSCs可以移植到骨缺损区域而没有人工支架来诱导骨再生。为了在临床环境中应用C-MSCs作为可靠的骨再生疗法，本研究旨在在无异种/无血清条件下产生C-MSCs，其可以发挥成功的骨再生特性并监测移植细胞与宿主之间的相互作用。将人骨髓来源的MSC在无异种/无血清培养基中培养。为了获得

间充质干细胞（MSC）/细胞外基质（ECM）复合物（C-MSC）的三维团块由细胞和自身产生的ECM组成。我们以前证明C-MSCs可以移植到骨缺损区域而没有人工支架来诱导骨再生。为了在临床环境中应用C-MSCs作为可靠的骨再生疗法，本研究旨在在无异种/无血清条件下产生C-MSCs，其可以发挥成功的骨再生特性并监测移植细胞与宿主之间的相互作用。

将人骨髓来源的MSC在无异种/无血清培养基中培养。为了获得C-MSC，使用微量移液管尖端刮擦在细胞片上形成的汇合细胞，然后撕下。将片材卷起以制成圆形的细胞团块。然后，将C-MSC移植到免疫缺陷小鼠颅骨缺损模型中。结果发现，C-MSC的移植以时间依赖性方式诱导骨再生。免疫荧光染色显示供体人细胞和宿主小鼠细胞均有助于骨重建。即使宿主小鼠细胞可以渗入缺损区域，脱细胞的C-MSC植入也不能诱导骨再生。

总之，这些发现表明，在无异种/无血清条件下产生的C-MSC可通过直接和间接成骨诱导骨再生。

原始出处：

Motoike S, Kajiya M, et al., Clumps of Mesenchymal Stem Cell/Extracellular Matrix Complexes Generated with Xeno-Free Conditions Facilitate Bone Regeneration via Direct and Indirect Osteogenesis. Int J Mol Sci. 2019 Aug 15;20(16). pii: E3970. doi: 10.3390/ijms20163970.

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    2019-12-19 h.y200607_23674418

    #间充质干细#

    0

  2. [GetPortalCommentsPageByObjectIdResponse(id=1815524, encodeId=f6a1181552417, content=<a href='/topic/show?id=ae359e231d4' target=_blank style='color:#2F92EE;'>#间充质干细#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=97231, encryptionId=ae359e231d4, topicName=间充质干细)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4b632500090, createdName=h.y200607_23674418, createdTime=Thu Dec 19 08:44:00 CST 2019, time=2019-12-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757188, encodeId=d2af1e5718891, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Thu Mar 26 14:44:00 CST 2020, time=2020-03-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1910644, encodeId=3928191064483, content=<a href='/topic/show?id=b45b426195e' target=_blank style='color:#2F92EE;'>#复合物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42619, encryptionId=b45b426195e, topicName=复合物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ad7f282, createdName=yang0210, createdTime=Sun Aug 09 03:44:00 CST 2020, time=2020-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1025328, encodeId=167410253281a, content=干细胞是热点，但是进入临床仍然需要时间和临床疗效验证哦, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=独孤立克, createdTime=Fri Sep 06 21:44:00 CST 2019, time=2019-09-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419330, encodeId=0ffa141933038, content=<a href='/topic/show?id=2e2e10182602' target=_blank style='color:#2F92EE;'>#骨缺损#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101826, encryptionId=2e2e10182602, topicName=骨缺损)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=51173492599, createdName=lfcmxl, createdTime=Thu Sep 05 12:44:00 CST 2019, time=2019-09-05, status=1, ipAttribution=)]
    2020-03-26 ms7899726347904398

    #基质#

    0

  3. [GetPortalCommentsPageByObjectIdResponse(id=1815524, encodeId=f6a1181552417, content=<a href='/topic/show?id=ae359e231d4' target=_blank style='color:#2F92EE;'>#间充质干细#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=97231, encryptionId=ae359e231d4, topicName=间充质干细)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4b632500090, createdName=h.y200607_23674418, createdTime=Thu Dec 19 08:44:00 CST 2019, time=2019-12-19, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1757188, encodeId=d2af1e5718891, content=<a href='/topic/show?id=103b421e0e8' target=_blank style='color:#2F92EE;'>#基质#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42170, encryptionId=103b421e0e8, topicName=基质)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=281337296540, createdName=ms7899726347904398, createdTime=Thu Mar 26 14:44:00 CST 2020, time=2020-03-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1910644, encodeId=3928191064483, content=<a href='/topic/show?id=b45b426195e' target=_blank style='color:#2F92EE;'>#复合物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=42619, encryptionId=b45b426195e, topicName=复合物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=ad7f282, createdName=yang0210, createdTime=Sun Aug 09 03:44:00 CST 2020, time=2020-08-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1025328, encodeId=167410253281a, content=干细胞是热点，但是进入临床仍然需要时间和临床疗效验证哦, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=独孤立克, createdTime=Fri Sep 06 21:44:00 CST 2019, time=2019-09-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1419330, encodeId=0ffa141933038, content=<a href='/topic/show?id=2e2e10182602' target=_blank style='color:#2F92EE;'>#骨缺损#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=101826, encryptionId=2e2e10182602, topicName=骨缺损)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=51173492599, createdName=lfcmxl, createdTime=Thu Sep 05 12:44:00 CST 2019, time=2019-09-05, status=1, ipAttribution=)]
    2020-08-09 yang0210

    #复合物#

    0

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    2019-09-06 独孤立克

    干细胞是热点，但是进入临床仍然需要时间和临床疗效验证哦

    0

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    2019-09-05 lfcmxl

    #骨缺损#

    0