ARCH PATHOL LAB MED：前列腺癌PTEN免疫组化检测前分析变量的影响

2019-05-09 不详网络

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磷酸酶和紧张素同源物(PTEN)是一种非常具有潜在应用价值的预后和潜在的预测前列腺癌的生物标志物。本研究的目的是评估分析前变量对经分析验证的全自动PTEN免疫组化检测的影响。

研究人员在Ventana 免疫染色系统上进行PTEN免疫组化。在良性前列腺组织中，通过数字图像分析评估不同条件下的免疫染色强度，并对前列腺肿瘤组织进行免疫染色。

研究显示，室温下延迟4小时或更长时间、4℃下延迟48小时或更长时间固定，福尔马林固定时间对免疫染色强度无明显影响。与其他固定剂相比，10%福尔马林染色强度最高。使用来自11个学术机构的协议处理PTEN缺失的肿瘤组织均可评估，得分相同。与组织块存放10年或更长时间的组织块相比，组织块存放不到10年的针活检的PTEN免疫染色更容易被评为不可评估的。在根治性前列腺切除术标本中这种效应不太明显，因为在保存了23年或更长时间后，这些标本的不可评估染色率很低。在免疫染色前将未染色的载玻片在室温下保存5年，与新切的玻片结果相同。对于12个肿瘤在3 个Ventana平台和2机构评估机器对机器变化发现变化极小，在77个肿瘤进行比较发现Ventana平台和徕卡仪器变化也比较小(κ= 0.926)。

自动PTEN免疫染色对前列腺中的大多数分析前变量都非常稳定，可用于多种分析前条件下的前列腺肿瘤组织。如果PTEN成为一个关键的预测生物标志物，这些数据将有助于指导检测的发展。

原始出处：

Liana B. Guedes, MD; Carlos L. Morais, MD;Effect of Preanalytic Variables on an Automated PTEN Immunohistochemistry Assay for Prostate Cancer

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2020-04-03 yb6560

#Pathol#

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2019-05-11 xiaogang317

#PTEN#

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2019-05-11 zhouqu_8

#免疫组化#

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2019-05-11 zz12341236

#PTEN免疫组化#

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2019-05-11 ms3994565386320060

#Med#

34 0
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2019-05-09 misszhang

前列腺癌相关研究，学习了，谢谢梅斯

59 0

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ARCH PATHOL LAB MED：前列腺癌PTEN免疫组化检测前分析变量的影响

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