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JCLA：快速鉴定不动杆菌属细菌的方法

2017-05-27 MedSci MedSci原创

不动杆菌属细菌PCR
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目前，不动杆菌属有50多种，其中四种亲缘关系很近，采用表型或基因型方法难以区分，如不动杆菌- 醋酸鲍曼不动杆菌复合体（ABC）。在流行病学方面，对不动杆菌属在种水平的正确识别是非常必要的。

近日，国际杂志Journal of Clinical Laboratory Analysis上在线发表一项关于基于gyrB基因的PCR检测方法快速鉴定鲍曼不动杆菌-醋酸钙不动杆菌的研究。

目前，不动杆菌属有50多种，其中四种亲缘关系很近，采用表型或基因型方法难以区分，如不动杆菌- 醋酸鲍曼不动杆菌复合体（ABC）。在流行病学方面，对不动杆菌属在种水平的正确识别是非常必要的。

本研究评估了利用gyrB基因的多重PCR以ITS 16S-23S片段的测序作为金标准来鉴定ABC的种类。研究纳入了2011年巴西南部三家医院鉴定为为醋酸鲍曼不动杆菌的分离株。

研究共获得了117份分离株，通过PCR检测gyrB基因，其中106份（90.6％）经确认为鲍曼不动杆菌，6份（5.1％）为A. nosocomialis，4份（3.4％）为A. pittii。只有一个分离株没有显示gyrB基因的PCR产物；通过ITS的测序将该分离株鉴定为A. genospecie。同时，研究人员还能从呼吸道（8 / 72.7％），血液（2/18.2％）和尿（1/9.1％）中得到非鲍曼不动杆菌分离物，表明这些种类可引起严重感染。

这些发现证明，gyrB的多重PCR是识别种级别的分离株的可行其简便的方法。

原始出处：

Aline B. Teixeira, Juliana Barin, et.al. PCR Assay Based on the gyrB Gene for Rapid Identification of Acinetobacter baumannii-calcoaceticus Complex at Specie Level.

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    2017-05-31 laoli

    谢谢分享，学习了！

    0

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    2017-05-29 130****4638

    学习了谢谢分享。

    0

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    2017-05-29 changjiu

    好文章，谢谢

    0

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    2017-05-28 laymankey

    感谢分享一下！！

    0

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    2017-05-28 天涯183

    非常好的文章，学习了，很受益

    0

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    2017-05-28 飛歌

    学习了很有用

    0

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    2017-05-28 杨利洪

    学习并分享了

    0

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    2017-05-27 有备才能无患

    这些发现证明，gyrB的多重PCR是识别种级别的分离株的可行其简便的方法。

    0