GUT: 甲基化循环DNA生物标志物可以用来监测转移性结直肠癌的治疗反应

2018-10-20 MedSci MedSci原创

有研究报道无细胞循环DNA(cfDNA)中的突变位点可以用来追踪结直肠癌(CRC)中的癌症复发。由于缺乏普遍突变的基因,该方法需要个性化的基因设计。相反,我们知道早期甲基化改变局限于确定的基因组位点,因此可以借此来进行群体研究,本项研究的目的是确定癌症特异性甲基化生物标志物是否可以在cfDNA(液体活检)中测量,以监测转移性CRC患者(mCRC)的治疗结果。

背景及目的 
有研究报道无细胞循环DNA(cfDNA)中的突变位点可以用来追踪直肠癌(CRC)中的癌症复发。由于缺乏普遍突变的基因,该方法需要个性化的基因设计。相反,我们知道早期甲基化改变局限于确定的基因组位点,因此可以借此来进行群体研究,本项研究的目的是确定癌症特异性甲基化生物标志物是否可以在cfDNA(液体活检)中测量,以监测转移性CRC患者(mCRC)的治疗结果。

方法
研究人员从149名CRC患者中,对细胞系的全基因组甲基化微阵列进行了检测,鉴定了五个癌症特异性甲基化基因座(EYA4,GRIA4,ITGA4,MAP3K14-AS1,MSC)。使用PCR来测量肿瘤组织DNA中这些基因的甲基化变化和来自mCRC的患者的cfDNA的差异

结果 
研究人员发现在所有肿瘤组织样品和156例 mCRC患者cfDNA样品中检测到至少一种标记物发生了甲基化(85.7%)。等离子标记患病率与基因位点之间的关系为71.4%--EYA4,68.5%--GRIA4,69.7%--ITGA4,69.1%--MAP3K14-AS1和65.1%--MSC。甲基化标记物的动态变化不受治疗类型的影响,并且与客观肿瘤反应和无进展存活相关。

结论 
上文所提到的五种基因甲基化变化可以用来监测不同治疗方案下液体活检中的肿瘤负荷动态,以评估CRC病人的治疗效果。

原始出处:

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  7. [GetPortalCommentsPageByObjectIdResponse(id=1937421, encodeId=8630193e4211d, content=<a href='/topic/show?id=c9fe50413e4' target=_blank style='color:#2F92EE;'>#循环DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50413, encryptionId=c9fe50413e4, topicName=循环DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9dd613, createdName=xxxx1049, createdTime=Sat Jan 26 09:07:00 CST 2019, time=2019-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1841312, encodeId=64041841312fd, content=<a href='/topic/show?id=18bf61953d3' target=_blank style='color:#2F92EE;'>#标志物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61953, encryptionId=18bf61953d3, topicName=标志物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6ab940, createdName=hukaixun, createdTime=Thu May 09 08:07:00 CST 2019, time=2019-05-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1386435, encodeId=d79d13864352b, content=<a href='/topic/show?id=f08293399f9' target=_blank style='color:#2F92EE;'>#转移性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93399, encryptionId=f08293399f9, topicName=转移性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1ac22500003, createdName=1249842em09(暂无昵称), createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453170, encodeId=d23314531e095, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1560779, encodeId=54081560e79ca, content=<a href='/topic/show?id=3c746936567' target=_blank style='color:#2F92EE;'>#生物标志#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69365, encryptionId=3c746936567, topicName=生物标志)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=63db15173977, createdName=zhu_jun9842, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1566788, encodeId=81ae1566e8823, content=<a href='/topic/show?id=3c746936567' target=_blank style='color:#2F92EE;'>#生物标志#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69365, encryptionId=3c746936567, topicName=生物标志)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=63db15173977, createdName=zhu_jun9842, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=350114, encodeId=4caa350114bf, content=学习了很多, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/22/c26524b0b4ca80000d38f0294262c002.jpg, createdBy=3cf52015303, createdName=苏小雨, createdTime=Mon Oct 22 00:42:32 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=350053, encodeId=b89d3500533f, content=学习了谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLbTp9nZtjwVrSZNxAXbAicnKQWnic2eBdJUQYhxO36XdkstSicr7FVF8p5ReH4omtmsbnLdvZLiaJJ1A/132, createdBy=8bff2327056, createdName=kafei, createdTime=Sun Oct 21 11:31:59 CST 2018, time=2018-10-21, status=1, ipAttribution=)]
    2018-10-22 苏小雨

    学习了很多

    0

  8. [GetPortalCommentsPageByObjectIdResponse(id=1937421, encodeId=8630193e4211d, content=<a href='/topic/show?id=c9fe50413e4' target=_blank style='color:#2F92EE;'>#循环DNA#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=58, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=50413, encryptionId=c9fe50413e4, topicName=循环DNA)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=9dd613, createdName=xxxx1049, createdTime=Sat Jan 26 09:07:00 CST 2019, time=2019-01-26, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1841312, encodeId=64041841312fd, content=<a href='/topic/show?id=18bf61953d3' target=_blank style='color:#2F92EE;'>#标志物#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=22, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61953, encryptionId=18bf61953d3, topicName=标志物)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=6ab940, createdName=hukaixun, createdTime=Thu May 09 08:07:00 CST 2019, time=2019-05-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1386435, encodeId=d79d13864352b, content=<a href='/topic/show?id=f08293399f9' target=_blank style='color:#2F92EE;'>#转移性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=93399, encryptionId=f08293399f9, topicName=转移性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=1ac22500003, createdName=1249842em09(暂无昵称), createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1453170, encodeId=d23314531e095, content=<a href='/topic/show?id=098ee8679ad' target=_blank style='color:#2F92EE;'>#结直肠#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=78679, encryptionId=098ee8679ad, topicName=结直肠)], attachment=null, authenticateStatus=null, createdAvatar=/v1.0.0/img/user_icon.png, createdBy=9ace5575596, createdName=crystal0564, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1560779, encodeId=54081560e79ca, content=<a href='/topic/show?id=3c746936567' target=_blank style='color:#2F92EE;'>#生物标志#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69365, encryptionId=3c746936567, topicName=生物标志)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=63db15173977, createdName=zhu_jun9842, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1566788, encodeId=81ae1566e8823, content=<a href='/topic/show?id=3c746936567' target=_blank style='color:#2F92EE;'>#生物标志#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69365, encryptionId=3c746936567, topicName=生物标志)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=63db15173977, createdName=zhu_jun9842, createdTime=Mon Oct 22 12:07:00 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=350114, encodeId=4caa350114bf, content=学习了很多, beContent=null, objectType=article, channel=null, level=null, likeNumber=40, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/05/22/c26524b0b4ca80000d38f0294262c002.jpg, createdBy=3cf52015303, createdName=苏小雨, createdTime=Mon Oct 22 00:42:32 CST 2018, time=2018-10-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=350053, encodeId=b89d3500533f, content=学习了谢谢, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/Q0j4TwGTfTLbTp9nZtjwVrSZNxAXbAicnKQWnic2eBdJUQYhxO36XdkstSicr7FVF8p5ReH4omtmsbnLdvZLiaJJ1A/132, createdBy=8bff2327056, createdName=kafei, createdTime=Sun Oct 21 11:31:59 CST 2018, time=2018-10-21, status=1, ipAttribution=)]
    2018-10-21 kafei

    学习了谢谢

    0