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1991 2
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Page 1
Identification, expression, and purification of DNA cytosine 5-methyltransferases with short recognition sequences.
Miura F, Miura M, Shibata Y, Furuta Y, Miyamura K, Ino Y, Bayoumi AMA, Oba U, Ito T. Miura F, et al. BMC Biotechnol. 2022 Nov 4;22(1):33. doi: 10.1186/s12896-022-00765-3. BMC Biotechnol. 2022. PMID: 36333700 Free PMC article.
CONCLUSIONS: In the current study, we established a procedure for producing active CC-methylating MTases and applied it to nucleosome occupancy and methylome sequencing to prove the usefulness of the enzyme for fine epigenetic mapping. ...
CONCLUSIONS: In the current study, we established a procedure for producing active CC-methylating MTases and applied it to nucleosome …
Epigenesis: the missing beat in biotechnology?
Strohman R. Strohman R. Biotechnology (N Y). 1994 Feb;12(2):156-64. doi: 10.1038/nbt0294-156. Biotechnology (N Y). 1994. PMID: 7764429 Review.
Only 2 percent of our total disease load is related to monogenic causality, and even here the final phenotype is modulated by many factors. Monogenic logic cannot, moreover, be applied to the 98 percent of our most important sources of premature disability and death. ...
Only 2 percent of our total disease load is related to monogenic causality, and even here the final phenotype is modulated by many factors. …
Alterations in the proteomic composition of Serratia marcescens in response to manganese (II).
Queiroz PS, Ruas FAD, Barboza NR, de Castro Borges W, Guerra-Sá R. Queiroz PS, et al. BMC Biotechnol. 2018 Dec 29;18(1):83. doi: 10.1186/s12896-018-0493-3. BMC Biotechnol. 2018. PMID: 30594179 Free PMC article.
To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics approach was applied to a potential Mn (II)-oxidizing Serratia marcescens strain cultivated in the absence and presence of Mn (II). . …
To gain insight into how bacteria respond to manganese (II) and identify the proteins involved in Mn (II) oxidation, the shotgun proteomics …
Production, purification and characterization of recombinant human R-spondin1 (RSPO1) protein stably expressed in human HEK293 cells.
Levin G, Koga BAA, Belchior GG, Carreira ACO, Sogayar MC. Levin G, et al. BMC Biotechnol. 2020 Jan 20;20(1):5. doi: 10.1186/s12896-020-0600-0. BMC Biotechnol. 2020. PMID: 31959207 Free PMC article.
CONCLUSION: Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading to the production of a purified, fully characterized and biologically active protein product to be applied in Tissue Engineering....
CONCLUSION: Therefore, a stable platform for the production and purification of recombinant hRSPO1 from HEK293 cells was generated, leading …
Identifying inhibitory compounds in lignocellulosic biomass hydrolysates using an exometabolomics approach.
Zha Y, Westerhuis JA, Muilwijk B, Overkamp KM, Nijmeijer BM, Coulier L, Smilde AK, Punt PJ. Zha Y, et al. BMC Biotechnol. 2014 Mar 21;14:22. doi: 10.1186/1472-6750-14-22. BMC Biotechnol. 2014. PMID: 24655423 Free PMC article.
BACKGROUND: Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellulosic biomass. An exometabolomics approach was applied to systematically identify inhibitors in lignocellulosic biomass hydrolysates. …
BACKGROUND: Inhibitors are formed that reduce the fermentation performance of fermenting yeast during the pretreatment process of lignocellu …
Characterization of purified and xerogel immobilized novel lignin peroxidase produced from Trametes versicolor IBL-04 using solid state medium of corncobs.
Asgher M, Iqbal HM, Irshad M. Asgher M, et al. BMC Biotechnol. 2012 Aug 3;12:46. doi: 10.1186/1472-6750-12-46. BMC Biotechnol. 2012. PMID: 22862820 Free PMC article.
Keeping in view the extensive industrial applications of lignin peroxidase (LiP), this study was performed to purify and characterize the LiP from an indigenous strain of Trametes versicolor IBL-04. Xerogel matrix enzyme immobilization technique was applied to improve the …
Keeping in view the extensive industrial applications of lignin peroxidase (LiP), this study was performed to purify and characterize the Li …
Amino terminal recognition by a CCR6 chemokine receptor antibody blocks CCL20 signaling and IL-17 expression via β-arrestin.
Gómez-Melero S, García-Maceira FI, García-Maceira T, Luna-Guerrero V, Montero-Peñalvo G, Túnez-Fiñana I, Paz-Rojas E. Gómez-Melero S, et al. BMC Biotechnol. 2021 Jul 5;21(1):41. doi: 10.1186/s12896-021-00699-2. BMC Biotechnol. 2021. PMID: 34225700 Free PMC article.
Finally, we determined by RT-qPCR that the expression of IL-17A in Th17 cells treated with 1C6 was inhibited. CONCLUSIONS: In the present study, we applied whole cell immunization for successfully obtain an antibody that is capable to neutralize hCCR6 signaling and to redu …
Finally, we determined by RT-qPCR that the expression of IL-17A in Th17 cells treated with 1C6 was inhibited. CONCLUSIONS: In the present st …
A novel synthetic trivalent single chain variable fragment (tri-scFv) construction platform based on the SpyTag/SpyCatcher protein ligase system.
Alam MK, Brabant M, Viswas RS, Barreto K, Fonge H, Ronald Geyer C. Alam MK, et al. BMC Biotechnol. 2018 Sep 10;18(1):55. doi: 10.1186/s12896-018-0466-6. BMC Biotechnol. 2018. PMID: 30200951 Free PMC article.
Use of the SpyTag/SpyCatcher protein ligase system allows Tri-scFvs to be rapidly constructed in a simple, modular manner, which can be easily applied to scFvs or other antibody fragments targeting other antigens....
Use of the SpyTag/SpyCatcher protein ligase system allows Tri-scFvs to be rapidly constructed in a simple, modular manner, which can be easi …
24 results