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Site-Specific Steric Control of SARS-CoV-2 Spike Glycosylation

期刊: BIOCHEMISTRY, 2021; 60 (27)

A central tenet in the design of vaccines is the display of native-like antigens in the elicitation of protective immunity. The abundance of N-linked ......

Effect of Surface Modification with Hydrocarbyl Groups on the Exocytosis of Nanoparticles

期刊: BIOCHEMISTRY, 2021; 60 (13)

Designing nanoparticles (NPs) with desirable cell type-specific exocytosis properties, say promoting their exocytosis from scavenging cell types (e.g.......

Liquid-Liquid Phase Separation in Biology: Specific Stoichiometric Molecular Interactions vs Promiscuous Interactions Mediated by Disordered Sequences

期刊: BIOCHEMISTRY, 2021; 60 (31)

Extensive studies in the past few years have shown that nonmembrane bound organelles are likely assembled via liquid-liquid phase separation (LLPS), a......

Studying Homo-oligomerization and Hetero-oligomerization of MDMX and MDM2 Proteins in Single Living Cells by Using In Situ Fluorescence Correlation Spectroscopy

期刊: BIOCHEMISTRY, 2021; 60 (19)

Protein oligomerization plays a very important role in many physiological processes. p53 acts as a key tumor suppressor by regulating cell cycle arres......

Insights into the Catalytic Mechanism of a Novel XynA and Structure-Based Engineering for Improving Bifunctional Activities

期刊: BIOCHEMISTRY, 2021; 60 (26)

Xylan and cellulose are the two major constituents of numerous types of lignocellulose. The bifunctional enzyme that exhibits xylanase/cellulase activ......

Cryo-EM Structure of the Photosynthetic LH1-RC Complex from Rhodospirillum rubrum

期刊: BIOCHEMISTRY, 2021; 60 (32)

Rhodospirillum (Rsp.) rubrum is one of the most widely used model organisms in bacterial photosynthesis. This purple phototroph is characterized by th......

Emerging Approaches to Functionalizing Cell Membrane-Coated Nanoparticles

期刊: BIOCHEMISTRY, 2021; 60 (13)

There has been significant interest in developing cell membrane-coated nanoparticles due to their unique abilities of biomimicry and biointerfacing. A......

Duplexes Formed by G(4)C(2) Repeats Contain Alternate Slow- and Fast-Flipping G center dot G Base Pairs

期刊: BIOCHEMISTRY, 2021; 60 (14)

Aberrant expansion of the hexanucleotide GGGGCC (or G(4)C(2)) repeat in the human C9ORF72 gene is the most common genetic factor found behind amyotrop......

Structural Insights into gp16 ATPase in the Bacteriophage phi 29 DNA Packaging Motor

期刊: BIOCHEMISTRY, 2021; 60 (11)

Biological motors, ubiquitous in living systems, convert chemical energy into different kinds of mechanical motions critical to cellular functions. Ge......

Common Pitfalls and Recommendations for Using a Turbidity Assay to Study Protein Phase Separation

期刊: BIOCHEMISTRY, 2021; 60 (32)

The turbidity assay is commonly exploited to study protein liquid-to-liquid phase separation (LLPS) or liquid-to-solid phase separation (LSPS) process......

Characterization of Enzymes Catalyzing the Formation of the Nonproteinogenic Amino Acid L-Dap in Capreomycin Biosynthesis

期刊: BIOCHEMISTRY, 2021; 60 (1)

Capreomycin (CMN) and viomycin (V10) are non-ribosomal peptide antituberculosis antibiotics, the structures of which contain four nonproteinogenic ami......

Curaxin-Induced DNA Topology Alterations Trigger the Distinct Binding Response of CTCF and FACT at the Single-Molecule Level

期刊: BIOCHEMISTRY, 2021; 60 (7)

The candidate anticancer drug curaxins can insert into DNA base pairs and efficiently inhibit the growth of various cancers. However, how curaxins alt......

Insight into the Therapeutic Selectivity of the Irreversible EGFR Tyrosine Kinase Inhibitor Osimertinib through Enzyme Kinetic Studies

期刊: BIOCHEMISTRY, 2020; 59 (14)

Osimertinib is a covalent, third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI) approved for treating non-small ce......

Identification of Binding Sites on Human Serum Albumin for Somapacitan, a Long-Acting Growth Hormone Derivative

期刊: BIOCHEMISTRY, 2020; 59 (14)

Somapacitan, a human growth hormone derivative that binds reversibly to albumin, was investigated for human serum albumin (HSA) and HSA domain binding......

Guest Sequence Can Influence RNA Encapsulation by an Engineered Cationic Protein Capsid

期刊: BIOCHEMISTRY, 2020; 59 (15)

Specific encapsulation of RNA in a cell is a challenging molecular recognition problem that has important implications for virology and drug delivery.......

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