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Stable overexpression of mutated PTEN in Chinese hamster ovary cells enhances their performance and therapeutic antibody production

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (9)

Chinese hamster ovary (CHO) cells with a high viable cell density (VCD), resilience to culture stress, and the capacity to continuously express recomb......

Surface engineering of biomaterials in orthopedic and dental implants: Strategies to improve osteointegration, bacteriostatic and bactericidal activities

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (7)

Background The success of biomedical implants in orthopedic and dental applications is usually limited due to insufficient bone-implant integration, a......

Fluorescence-based screening for engineered aldo-keto reductase KmAKR with improved catalytic performance and extended substrate scope

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (9)

Background Aldo-keto reductases-catalyzed transformations of ketones to chiral alcohols have become an established biocatalytic process step in the ph......

Optimization of CRISPR-Cas9 through promoter replacement and efficient production of L-homoserine in Corynebacterium glutamicum

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (8)

Background: Corynebacterium glutamicum is an important chassis for industrial applications. The low efficiency of commonly used genome editing methods......

An improved lentiviral system for efficient expression and purification of beta-defensins in mammalian cells

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (9)

beta-Defensins are a family of conserved small cationic antimicrobial peptides with different significant biological functions. The majority of mammal......

Conversion of five proluciferin esters by human cytochrome P450 enzymes

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (7)

Background Probe substrates are an important tool for activity monitoring of human drug metabolizing enzymes such as cytochromes P450 (CYPs). Brief Me......

Recombineering facilitates the discovery of natural product biosynthetic pathways in Pseudomonas parafulva

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (8)

Microbial natural products among other functions they play a vital role in the disease prevention in humans, animals and plants. Pseudomonas parafulva......

Optimization of culture conditions for human bone marrow-derived mesenchymal stromal cell expansion in macrocarrier-based Tide Motion system

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (7)

Background: With high cell doses required for mesenchymal stromal cell (MSC) clinical trials, there is a need to upgrade technologies that facilitate ......

Spatial transcriptomics and proteomics technologies for deconvoluting the tumor microenvironment

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (9)

The tumor microenvironment (TME) harbors heterogeneous contents and plays critical roles in tumorigenesis, metastasis, and drug resistance. Therefore,......

Western equine encephalitis virus virus-like particles from an insect cell-baculovirus system elicit the strong immune responses in mice

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (8)

Western equine encephalitis virus (WEEV) causes lethal encephalitis in humans and equines, and it poses a serious public health threat in many countri......

Recent advances in engineering of microbial cell factories for intelligent pH regulation and tolerance

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (9)

pH regulation is a serious concern in the industrial fermentation process as pH adjustment heavily utilizes acid/base and pollutes the environment. Un......

Engineering the oleaginous yeast Yarrowia lipolytica for beta-farnesene overproduction

期刊: BIOTECHNOLOGY JOURNAL, 2021; 16 (7)

beta-farnesene is a sesquiterpenoid with various industrial applications which is now commercially produced by a Saccharomyces cerevisiae strain obtai......

Engineering a thermostable chondroitinase for production of specifically distributed low-molecular-weight chondroitin sulfate

期刊: BIOTECHNOLOGY JOURNAL, ; ()

Chondroitinase ABC I (csABC I) has attracted intensive attention because of its great potential in heparin refining and the enzymatic preparation of l......

Metabolomic analysis improves bioconversion of methanol to isobutanol in Methylorubrum extorquens AM1

期刊: BIOTECHNOLOGY JOURNAL, ; ()

Background Methylorubrum extorquens AM1 can be engineered to convert methanol to value-added chemicals. Most of these chemicals derive from acetyl-CoA......

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