加载中........
×

文献资料信息综合查询

关键词(只能英文): 支持多关键词搜索

注册会员

以下是搜索结果
分享到:
PARASITOLOGY RESEARCH, 2009; 105 (4): 907

Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA

Chitimia L; Lin RQ; Cosoroaba I; Braila P; Song HQ; Zhu XQ
Zhu, XQ, S China Agr Univ, Coll Vet Med, 483 Wushan St, Guangzhou 510642, Guangdong, Peoples R China.
分享到:

Abstract


Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA | | In the present study, four hard tick species and one soft tick species, namely, Dermacentor marginatus, Haemaphysalis punctata, Haemaphysalis parva, Ixodes ricinus, and Dermanyssus gallinae, from south-western Romania were characterized genetically by the first (ITS-1) and second (ITS-2) internal transcribed spacers (ITS) of nuclear ribosomal DNA (rDNA), using a hard tick, Haemaphysalis longicornis, from China for comparative purposes. The ITS rDNA was amplified by polymerase chain reaction (PCR) and sequenced from individual ticks. The lengths of the ITS-1 sequences were 238-1819 bp, and the lengths of ITS-2 were 137-1695 bp, respectively, for all ticks sequenced. While sequence variation within a hard tick species was 0-1.5%, nucleotide differences between hard tick species ranged 2-25.2%, indicating that ITS rDNA sequences provide genetic markers for the differentiation of hard ticks from Romania. Hence, a PCR-linked restriction fragment length polymorphism approach was developed for their unequivocal differentiation based on ITS-1 rDNA. This is the first characterization of ticks from Romania using a genetic approach, which provides the foundation for further studies on ticks in Romania and has implications for studying the population genetic structure of the Romanian ticks and for identification and differentiation of closely related ticks.

PARASITOLOGY RESEARCH 影响因子:2.027    (投稿经验 | MedSci指数 | 杂志信息 )
原文下载:PDF文件下载

原文查看:Molecular characterization of hard and soft ticks from Romania by sequences of the internal transcribed spacers of ribosomal DNA


web对话
live chat

扫码领取IF曲线

IF连续增长的期刊