Molecular diversity of Chinese Cucurbita moschata germplasm collections detected by AFLP markers
Wu, JX; Chang, ZJ; Wu, QS; Zhan, HX; Xie, SL
Chang, ZJ (reprint author), Shanxi Acad Agr Sci, Inst Crop Sci, 185 Taiyu Rd, Taiyuan 030032, Shanxi, Peoples R China
Cucurbita moschata is an important vegetable crop. Although a total of 1032 landraces of C. moschata are maintained in China, little is known about their genetic diversity. Molecular characterization is needed to facilitate the use of this C. moschata germplasm collection in breeding. Seventy-four Chinese accessions and 15 accessions from other countries were selected for evaluation based upon variation in fruit traits and geographical origin of molecular diversity with AFLP analysis. Nine pairs of EcoRI/Msel primers produced 500 fragments, of which 75.57% were polymorphic, indicating a high degree of diversity. The accessions from China were classified into two clusters, which were clearly differentiated from the accessions originating from Mexico, Guatemala, Honduras, and Ecuador. Chinese group genetically more closely related to other Asian countries group (India and Japan). In general, the accessions from the Americas had a greater number of unique loci than those from China. The differences are probably due to a limited number of introductions and genetic drift. The Americas are the center of origin of C. moschata and therefore more diverse. With AFLP analysis, the accessions did not clearly group according to fruit shape; however, sub-clusters exist in acorn- and dumbbell-shaped accessions. The assessment of genetic distance, along with some unique traits among the different genotypes, could be useful in further genetic studies and the selection of the most adequate accessions for use in breeding programs. (c) 2010 Elsevier B.V. All rights reserved.
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