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Stem cells：应用低浓度的去铁敏（DFO）预处理MSCs，其有效性足以替代低氧培养

2018-03-27 MedSci MedSci原创

MSCs再生疗法去铁敏低氧培养
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间充质干细胞（MSCs）常用于再生医学，但其治疗效果非常依赖于培养环境。低氧培养可维持干细胞处于未分化的状态，但费用高而且难以操作。去铁敏（DFO），一种低氧模拟试剂，Koichi Fujisawa等人现通过分析DFO诱导的MSCs的代谢改变与低氧培养的MSCs的代谢变化进行对比，来评估DFO替代低氧培养的效果。低浓度的DFO可降低线粒体活性并减少细胞凋亡，预处理MSC的效果媲美低氧培养。此外，代

间充质干细胞（MSCs）常用于再生医学，但其治疗效果非常依赖于培养环境。低氧培养可维持干细胞处于未分化的状态，但费用高而且难以操作。

去铁敏（DFO），一种低氧模拟试剂，Koichi Fujisawa等人现通过分析DFO诱导的MSCs的代谢改变与低氧培养的MSCs的代谢变化进行对比，来评估DFO替代低氧培养的效果。

低浓度的DFO可降低线粒体活性并减少细胞凋亡，预处理MSC的效果媲美低氧培养。此外，代谢组学分析显示低氧治疗和DFO管理呈现相似的代谢模式，除了嘌呤、嘧啶和TCA循环相关代谢。

综上所述，低浓度的DFO，或可替代低氧培养。该发现为以后利用MSCs的再生疗法的发展奠定基础。

原始出处：


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    2018-10-08 zhaozhouchifen

    #Cell#

    0

  2. 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    2018-09-03 维他命

    #CEL#

    0

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    2018-09-23 hb2008ye

    #stem cell#

    0

  4. 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encryptionId=46e716e82a6, topicName=stem cells)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3883266, createdName=yykkxiaodou, createdTime=Wed Mar 06 18:11:00 CST 2019, time=2019-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406700, encodeId=38cb1406e009e, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=44, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1482112, encodeId=5881148211264, content=<a href='/topic/show?id=8cc416e78f3' target=_blank style='color:#2F92EE;'>#STEM#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16778, encryptionId=8cc416e78f3, topicName=STEM)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d1067833268, createdName=12498bb3m92(暂无昵称), createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544517, encodeId=2900154451e76, content=<a href='/topic/show?id=ac3d6549e75' target=_blank style='color:#2F92EE;'>#浓度#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=21, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=65497, encryptionId=ac3d6549e75, topicName=浓度)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=医者仁心, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2019-03-06 yykkxiaodou

    #stem cells#

    0

  5. 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time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-29 huanbaofeng

    #MSC#

    0

  6. 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time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-29 12498bb3m92(暂无昵称)

    #STEM#

    0

  7. 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encryptionId=46e716e82a6, topicName=stem cells)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=3883266, createdName=yykkxiaodou, createdTime=Wed Mar 06 18:11:00 CST 2019, time=2019-03-06, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1406700, encodeId=38cb1406e009e, content=<a href='/topic/show?id=099d122e1cd' target=_blank style='color:#2F92EE;'>#MSC#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=44, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12271, encryptionId=099d122e1cd, topicName=MSC)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83epVh4QoCj2A7xBBEjh8q7ia2BsLkylnuQ15ia2MDzvr28XR0CVvBPpFXTlB1mprG7WgBb06gvn4Eia5Q/132, createdBy=55e02571190, createdName=huanbaofeng, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1482112, encodeId=5881148211264, 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time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-29 医者仁心

    #浓度#

    0

  8. 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time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-29 124988c7m101暂无昵称

    #cells#

    0

  9. 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time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1614990, encodeId=cc80161499025, content=<a href='/topic/show?id=d74a44e2e7' target=_blank style='color:#2F92EE;'>#cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4472, encryptionId=d74a44e2e7, topicName=cells)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=659819575782, createdName=124988c7m101暂无昵称, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1625911, encodeId=a1171625911d9, content=<a href='/topic/show?id=7435122e3c3' target=_blank style='color:#2F92EE;'>#MSCs#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=12273, encryptionId=7435122e3c3, topicName=MSCs)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-29 zhanfl

    #MSCs#

    0

  10. 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authenticateStatus=null, createdAvatar=null, createdBy=18ac20929511, createdName=zhanfl, createdTime=Thu Mar 29 12:11:00 CST 2018, time=2018-03-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=300272, encodeId=37f23002e2a3, content=谢谢分享学习了, beContent=null, objectType=article, channel=null, level=null, likeNumber=56, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM7GibvIBibibnnnvpajk2stlDF5oichYahI6Bia8uxeTBFoPbdoHWebyXf9eal9FNgUF2lNcrwoZicM3BvQ/0, createdBy=a3742066385, createdName=戒馋，懒，贪, createdTime=Tue Mar 27 21:34:04 CST 2018, time=2018-03-27, status=1, ipAttribution=)]
    2018-03-27 戒馋，懒，贪

    谢谢分享学习了

    0

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