J Endod:对新型包含氢氧化钙的环氧树脂类牙髓封闭剂的细胞毒性和生物相容性评估

2017-10-21 lishiting MedSci原创

目前临床上拥有多种牙髓封闭剂材料,但对理想封闭剂的探索却从未停止过。这篇研究的目的是评估一种含有氢氧化钙的新型环氧树脂类牙髓封闭剂-Sealer Plus的细胞毒性和生物相容性。AH Plus, Endofill和SimpliSeal牙髓封闭剂用来做对照。

目前临床上拥有多种牙髓封闭剂材料,但对理想封闭剂的探索却从未停止过。这篇研究的目的是评估一种含有氢氧化钙的新型环氧树脂类牙髓封闭剂-Sealer Plus的细胞毒性和生物相容性。AH Plus, Endofill和SimpliSeal牙髓封闭剂用来做对照。

研究收集和培养L929成纤维细胞,并通过MTT法对封闭剂浸膏剂在6, 24, 48和72小时的细胞毒性进行检测。研究用导管盛装实验材料或空管做对照,分别植入20只大鼠的皮下组织。在7和30天后,处死实验大鼠,取出导管及周围组织进行组织学分析。所得数据进行统计学分析(P < .05)。

结果显示,未稀释的Sealer Plus在6小时比其他未稀释的浸润膏展现出更小的细胞毒性(P < .05),24小时后Sealer Plus 浸润膏的细胞活力要更高(P < .05)。在48小时,未稀释和稀释一半的Sealer Plus浸润膏的细胞毒性更小(P < .05)。在72小时,未稀释和稀释一半的Sealer Plus的细胞活力比其他封闭剂明显增强(P < .05)。7天时,Endofill和SimpliSeal的感染情况比对照组和Sealer Plus更明显(P < .05);而AH Plus具有中等程度的炎症(P > .05)。30天时,对照组, Sealer Plus和AH Plus的感染程度相对较低(P < .05)。另外,除了SimpliSeal,其他封闭剂的纤维囊在7天时较厚,30天时较薄。

结论:总的来说,Sealer Plus可以增强细胞的活力,并且比其他封闭剂具有更好的生物相容性。

原始出处

Cintra LTA, Benetti F, et al. Evaluation of the Cytotoxicity and Biocompatibility of New Resin Epoxy-based Endodontic Sealer Containing Calcium Hydroxide. J Endod. 2017 Oct 9. pii: S0099-2399(17)30905-6. doi: 10.1016/j.joen.2017.07.016.

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    2018-04-12 jxrzshh
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  7. [GetPortalCommentsPageByObjectIdResponse(id=1798178, encodeId=53761e9817867, content=<a href='/topic/show?id=02e068505b7' target=_blank style='color:#2F92EE;'>#环氧树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=68505, encryptionId=02e068505b7, topicName=环氧树脂)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/Bnia2EtH2IvyBtniaWHPQOYAicsN3IAL1ZuPV7qyALcdCiawRJdEiafMxWTxzERQW7eeKg8HfCKCRK2CJicy073C4RJg/132, createdBy=464b2500186, createdName=ms1948154235210413, createdTime=Wed Mar 07 18:44:00 CST 2018, time=2018-03-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669610, encodeId=18b1166961095, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Thu Apr 12 09:44:00 CST 2018, time=2018-04-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1443036, encodeId=02ce1443036f6, content=<a href='/topic/show?id=674546e8323' target=_blank style='color:#2F92EE;'>#封闭剂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=46783, encryptionId=674546e8323, topicName=封闭剂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b88a5021004, createdName=12498dd9m12暂无昵称, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529512, encodeId=617c15295126e, content=<a href='/topic/show?id=a02ce793707' target=_blank style='color:#2F92EE;'>#细胞毒性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77937, encryptionId=a02ce793707, topicName=细胞毒性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=10b211951227, createdName=appleandpeer, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1569920, encodeId=b528156992037, content=<a href='/topic/show?id=e14a6395908' target=_blank style='color:#2F92EE;'>#氢氧化钙#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63959, encryptionId=e14a6395908, topicName=氢氧化钙)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f8f315601930, createdName=tongyongming, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1586629, encodeId=5cca158662930, content=<a href='/topic/show?id=155269406ec' target=_blank style='color:#2F92EE;'>#生物相容性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69406, encryptionId=155269406ec, topicName=生物相容性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c6cb17357412, createdName=bnurmamat, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1627561, encodeId=b9fb162e56138, content=<a href='/topic/show?id=cea46199eba' target=_blank style='color:#2F92EE;'>#树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61997, encryptionId=cea46199eba, topicName=树脂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=378b21121654, createdName=chenshujs, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=254939, encodeId=b7252549391b, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sat Oct 21 22:33:12 CST 2017, time=2017-10-21, status=1, ipAttribution=)]
    2017-10-23 chenshujs
  8. [GetPortalCommentsPageByObjectIdResponse(id=1798178, encodeId=53761e9817867, content=<a href='/topic/show?id=02e068505b7' target=_blank style='color:#2F92EE;'>#环氧树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=33, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=68505, encryptionId=02e068505b7, topicName=环氧树脂)], attachment=null, authenticateStatus=null, createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/Bnia2EtH2IvyBtniaWHPQOYAicsN3IAL1ZuPV7qyALcdCiawRJdEiafMxWTxzERQW7eeKg8HfCKCRK2CJicy073C4RJg/132, createdBy=464b2500186, createdName=ms1948154235210413, createdTime=Wed Mar 07 18:44:00 CST 2018, time=2018-03-07, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1669610, encodeId=18b1166961095, content=<a href='/topic/show?id=c7ca6e660ab' target=_blank style='color:#2F92EE;'>#牙髓#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=30, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67660, encryptionId=c7ca6e660ab, topicName=牙髓)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=5eeb26562774, createdName=jxrzshh, createdTime=Thu Apr 12 09:44:00 CST 2018, time=2018-04-12, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1443036, encodeId=02ce1443036f6, content=<a href='/topic/show?id=674546e8323' target=_blank style='color:#2F92EE;'>#封闭剂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=31, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=46783, encryptionId=674546e8323, topicName=封闭剂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b88a5021004, createdName=12498dd9m12暂无昵称, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1529512, encodeId=617c15295126e, content=<a href='/topic/show?id=a02ce793707' target=_blank style='color:#2F92EE;'>#细胞毒性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77937, encryptionId=a02ce793707, topicName=细胞毒性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=10b211951227, createdName=appleandpeer, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1569920, encodeId=b528156992037, content=<a href='/topic/show?id=e14a6395908' target=_blank style='color:#2F92EE;'>#氢氧化钙#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=36, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=63959, encryptionId=e14a6395908, topicName=氢氧化钙)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f8f315601930, createdName=tongyongming, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1586629, encodeId=5cca158662930, content=<a href='/topic/show?id=155269406ec' target=_blank style='color:#2F92EE;'>#生物相容性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=69406, encryptionId=155269406ec, topicName=生物相容性)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=c6cb17357412, createdName=bnurmamat, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1627561, encodeId=b9fb162e56138, content=<a href='/topic/show?id=cea46199eba' target=_blank style='color:#2F92EE;'>#树脂#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=61997, encryptionId=cea46199eba, topicName=树脂)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=378b21121654, createdName=chenshujs, createdTime=Mon Oct 23 11:44:00 CST 2017, time=2017-10-23, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=254939, encodeId=b7252549391b, content=学习了.涨知识, beContent=null, objectType=article, channel=null, level=null, likeNumber=52, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://img.medsci.cn/20220115/46bcf39c32de4aa6b45c5f9d66c8ee77/6cb4a20c55bb4b7691122f47747bfca2.jpg, createdBy=9dad1662329, createdName=1ddf0692m34(暂无匿称), createdTime=Sat Oct 21 22:33:12 CST 2017, time=2017-10-21, status=1, ipAttribution=)]
    2017-10-21 1ddf0692m34(暂无匿称)

    学习了.涨知识

    0

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