J Periodon Res:晚期糖基化终末产物增加人口腔上皮细胞脂钙蛋白2的表达

2020-03-31 网络 网络

糖尿病(DM)是牙周病的危险因素,会加重牙周病的严重程度。 糖尿病并发症的主要因素之一是晚期糖基化终末产物(AGEs)在牙周组织中积累并引起炎症反应。

糖尿病(DM)是牙周病的危险因素,会加重牙周病的严重程度。 糖尿病并发症的主要因素之一是晚期糖基化终末产物(AGEs)在牙周组织中积累并引起炎症反应。 脂钙蛋白2(LCN2)是一种抗菌肽和炎症相关因子, DM中LCN2水平升高。 本研究探讨了牙龈卟啉单胞菌的脂多糖(Pg-LPs) 和AGEs对人口腔上皮细胞(TR146细胞)LCN2表达的影响,及其分泌的LCN2在DMB+牙周炎中的作用。

TR146细胞与AGEs(AGE2)或Pg-LPS共培养,BSA作为对照,检测细胞活力。从上皮细胞培养物中制备条件培养基和细胞裂解物,用Western blotting和ELISA检测LCN2、RAGE、IL-6、MAPK和NF-κB的表达水平。从AGEs处理的TR146细胞和分化的HL-60(D-HL-60)细胞中分离RNA,采用实时定量PCR检测LCN2和白细胞介素-6(IL-6)mRNAs的表达。用RAGE-和LCN2-siRNAs(siRNA,siLCN2)转染上皮细胞,检测AGEs诱导后LCN2的表达。将D-HL-60细胞与siLCN2转染的TR146细胞共培养,并用AGEs处理,检测D-HL-60细胞中IL-6 mRNA的表达和细胞迁移。

结果发现,AGEs提高了口腔上皮细胞LCN2和IL-6的表达水平。siRAGE和RAGE中和抗体抑制AGEs诱导的LCN2表达。AGEs刺激上皮细胞ERK、p38和NF-κB的磷酸化,其抑制剂抑制了AGEs诱导的LCN2的表达。 相反,在共培养实验中,Pg-LPS组没有显着增加表达Toll样受体2的TR146细胞中LCN2的水平,AGEs诱导的LCN2抑制D-HL-60细胞中IL-6 mRNA的表达,上皮细胞敲除LCN2抑制了HL-60细胞迁移。

这些结果表明,AGEs通过RAGE、MAPK和NF-κB信号通路增加了口腔上皮细胞LCN2的表达,分泌的LCN2可能影响DM+牙周炎的病理状况。

原始出处:

Rie Kido, Advanced glycation end‐products increase lipocalin 2 expression in human oral epithelial cells. Journal of periodontal research, 2020 March. Doi: 10.1111/jre.12741

版权声明:
本网站所有内容来源注明为“梅斯医学”或“MedSci原创”的文字、图片和音视频资料,版权均属于梅斯医学所有。非经授权,任何媒体、网站或个人不得转载,授权转载时须注明来源为“梅斯医学”。其它来源的文章系转载文章,或“梅斯号”自媒体发布的文章,仅系出于传递更多信息之目的,本站仅负责审核内容合规,其内容不代表本站立场,本站不负责内容的准确性和版权。如果存在侵权、或不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。
在此留言
评论区 (3)
#插入话题
  1. [GetPortalCommentsPageByObjectIdResponse(id=1728375, encodeId=30051e2837511, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Mon Jun 29 05:23:23 CST 2020, time=2020-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1318314, encodeId=5491131831414, content=<a href='/topic/show?id=b5dde6644b1' target=_blank style='color:#2F92EE;'>#糖基化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76644, encryptionId=b5dde6644b1, topicName=糖基化)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43cb463, createdName=lfyang, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1533908, encodeId=5a80153390882, content=<a href='/topic/show?id=4be9200308c' target=_blank style='color:#2F92EE;'>#上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20030, encryptionId=4be9200308c, topicName=上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3a5d12492718, createdName=lizhou0208, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=)]
    2020-06-29 feather89
  2. [GetPortalCommentsPageByObjectIdResponse(id=1728375, encodeId=30051e2837511, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Mon Jun 29 05:23:23 CST 2020, time=2020-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1318314, encodeId=5491131831414, content=<a href='/topic/show?id=b5dde6644b1' target=_blank style='color:#2F92EE;'>#糖基化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76644, encryptionId=b5dde6644b1, topicName=糖基化)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43cb463, createdName=lfyang, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1533908, encodeId=5a80153390882, content=<a href='/topic/show?id=4be9200308c' target=_blank style='color:#2F92EE;'>#上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20030, encryptionId=4be9200308c, topicName=上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3a5d12492718, createdName=lizhou0208, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=)]
    2020-04-02 lfyang
  3. [GetPortalCommentsPageByObjectIdResponse(id=1728375, encodeId=30051e2837511, content=<a href='/topic/show?id=986913980dd' target=_blank style='color:#2F92EE;'>#PE#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=27, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=13980, encryptionId=986913980dd, topicName=PE)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f06c33759375, createdName=feather89, createdTime=Mon Jun 29 05:23:23 CST 2020, time=2020-06-29, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1318314, encodeId=5491131831414, content=<a href='/topic/show?id=b5dde6644b1' target=_blank style='color:#2F92EE;'>#糖基化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=0, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76644, encryptionId=b5dde6644b1, topicName=糖基化)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=43cb463, createdName=lfyang, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1533908, encodeId=5a80153390882, content=<a href='/topic/show?id=4be9200308c' target=_blank style='color:#2F92EE;'>#上皮细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=38, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=20030, encryptionId=4be9200308c, topicName=上皮细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=3a5d12492718, createdName=lizhou0208, createdTime=Thu Apr 02 14:23:23 CST 2020, time=2020-04-02, status=1, ipAttribution=)]

相关资讯

Diabetologia: 做好这四点,可防75%糖尿病!华中科大学者研究

健康的生活方式,即保持健康体重、健康饮食、每天至少锻炼30分钟、不吸烟、不喝酒,是防治糖尿病的基石。

MedRxiv:高血压和糖尿病导致「新冠」患者痊愈速度更慢!

新冠肺炎疫情在中国已经得到了有效防控,但在其他许多国家正呈现迅猛的爆发趋势,医疗资源的短缺问题迫在眉睫。病毒的彻底清除是衡量新冠患者痊愈的金标准。那么患者体内病毒清除平均需要多长时间?

Cell Death Dis:抑制TGF-β/Smad3信号通路可阻断胰岛β细胞凋亡

TGF-β/Smad3信号通路在胰腺β细胞凋亡过程中具有重要作用

Nature Food: 精制淀粉和糖要为全球肥胖和糖尿病负责!

近期,Nature子刊一篇文章指出,要解决目前肥胖和2型糖尿病流行,要从食物入手,应当吃具有生物活性的食物,例如水果、坚果、种子、非淀粉类蔬菜、豆类、植物油、全谷物、酸奶和鱼,而少吃加工食物。

Prim Care Diabetes:河南学者研究称血压高预示未来患糖尿病!

河南学者联合深圳学者发表基于中国农村人口的大样本队列研究发现,随着平均动脉压的增加,女性患2型糖尿病的风险增加。平均动脉压 = 1/3×收缩压 + 2/3×舒张压。