Biosensors and Bioelectronics:45分钟快速检测新冠,王永明/倪挺/郑琰团队开发出基于CRISPR的新冠快速检测方法

2020-10-29 Bio生物世界 Bio生物世界

随着冬季的到来,全球正面临第二波新冠肺炎(COVID-19)大流行,简便快速的核酸检测手段对疫情防控十分重要。临床上使用最多的检测技术是实时定量RT-PCR技术。

随着冬季的到来,全球正面临第二波新冠肺炎COVID-19)大流行,简便快速的核酸检测手段对疫情防控十分重要。临床上使用最多的检测技术是实时定量RT-PCR技术,但该技术需要昂贵的设备,检测费用高,时间长。

2020年10月26日,复旦大学王永明团队、倪挺团队、郑琰团队联合杭州市第一人民医院,在 Biosensors and Bioelectronics 杂志在线发表了题为:opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection 的论文。

研究团队开发出了一套名为“ opvCRISPR”的新型冠状病毒检测系统,具有检测时间短、灵敏度高、结果可视化的特点。

将逆转录环介导等温扩增技术(RT-LAMP)及Cas12a剪切整合到一个系统中,能够在45分钟内检测接近单个分子的样本,诊断结果与实时定量RT-PCR测定结果一致。

图1:opvCRISPR检测流程

图2:opvCRISPR 方法与RT-PCR方法在SARS-CoV-2样本中的检测结果

王瑞博士与钱春艳博士是论文的共同第一作者,王永明研究员、倪挺研究员、郑琰研究员为论文的共同通讯作者。

原始出处:
RuiWanga,ChunyanQianbc,YananPang,et al.opvCRISPR: One-pot visual RT-LAMP-CRISPR platform for SARS-cov-2 detection.Biosensors and Bioelectronics.Volume 172, 15 January 2021, 112766https://doi.org/10.1016/j.bios.2020.112766

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    2020-11-22 sunylz
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    2021-09-27 hxj0117
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    2020-10-31 yuandd
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    2020-10-29 公卫新人

    新冠肺炎,疫情何时才能消失

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