J Periodontal Res：胱硫醚γ-裂解酶加重小鼠咬合创伤模型的牙周损伤

2020-04-30 网络网络

尽管咬合创伤（TO）对牙周组织的影响，和胱硫醚γ-裂解酶（Cth）基因在骨平衡调控中的作用已经有所研究，但TO是否会加重牙周组织破坏以及Cth在咬合创伤中的确切作用还没有达成共识。

尽管咬合创伤（TO）对牙周组织的影响和胱硫醚γ-裂解酶（Cth）基因在骨平衡调控中的作用已经有所研究，但TO是否会加重牙周组织破坏以及Cth在咬合创伤中的确切作用还没有达成共识。因此，本研究旨在探讨TO对牙周组织的影响及Cth基因的作用。

本研究纳入80只8周龄的C57BL/6野生型（WT）小鼠和Cth基因敲除（Cth-/-）小鼠。在第1周、2周和3周，用复合树脂粘接左上颌磨牙建立TO模型。通过micro-CT、H&E染色和酒石酸抗酸磷酸酶(TRAP)染色评估牙周的形态学和组织学变化。实时聚合酶链反应（qPCR）分析成骨细胞相关基因。

结果发现，随着建模时间的增加，TO模型中的牙槽骨高度降低，骨吸收面积扩大，牙周膜间隙增加，破骨细胞数量增加，破骨细胞相关的基因包括Ctsk、Mmp9、Rank、Trap和Rankl/Opg在建模1周后上调，Cth基因及其蛋白CTH的表达上调。经过1，2或3周的建模后，与Cth-/-小鼠相比，WT小鼠的牙槽骨吸收，牙周膜间隙增加，破骨细胞数量增加，和破骨细胞相关基因Ctsk，Rank和Rankl/Opg的水平升高更加明显。

因此，TO可导致牙槽骨高度降低和牙周膜形态紊乱，其严重程度随时间的推移而增加。Cth能够加重TO引起的牙周损伤。

原文出处：

Liyuan Chen， Cystathionine gamma‐lyase aggravates periodontal damage in traumatic occlusion mouse models， journal of periodontal research， 2020， doi：10.1111/jre.12753

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2020-12-11 Eleven11

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2020-06-20 41514494@qq.com

#咬合创伤#

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likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Apr 30 23:14:03 CST 2020, time=2020-04-30, status=1, ipAttribution=)]
2020-07-04 12498cbcm10(暂无昵称)

#裂解酶#

28 0
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2020-10-15 ms1343860338762272

#胱硫醚γ-裂解酶#

24 0
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likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Apr 30 23:14:03 CST 2020, time=2020-04-30, status=1, ipAttribution=)]
2020-05-02 ms920641618706883

#创伤#

26 0
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likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Apr 30 23:14:03 CST 2020, time=2020-04-30, status=1, ipAttribution=)]
2020-05-02 ms3046638856685384

#损伤#

24 0
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likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Apr 30 23:14:03 CST 2020, time=2020-04-30, status=1, ipAttribution=)]
2020-05-02 医者仁心

#牙周#

25 0
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createdAvatar=https://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eoNX3lUOlDKGBUEPNwZx0nibmdcFyeibnX4WMhUSCU2REpGRWtSmnT2CCs0dA5waxvJknYDxwtYnIcQ/132, createdBy=294e2500218, createdName=ms3046638856685384, createdTime=Sat May 02 11:14:03 CST 2020, time=2020-05-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1451240, encodeId=4a78145124020, content=<a href='/topic/show?id=f0476e49582' target=_blank style='color:#2F92EE;'>#牙周#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67495, encryptionId=f0476e49582, topicName=牙周)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=医者仁心, createdTime=Sat May 02 11:14:03 CST 2020, time=2020-05-02, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1047801, encodeId=c063104e8015f, content=梅斯里提供了很多疾病的模型计算公式，赞一个！, beContent=null, objectType=article, channel=null, level=null, likeNumber=54, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f0620, createdName=CHANGE, createdTime=Thu Apr 30 23:14:03 CST 2020, time=2020-04-30, status=1, ipAttribution=)]
2020-04-30 CHANGE

梅斯里提供了很多疾病的模型计算公式，赞一个！

54 0

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