Clin Oral Implants Res:亲水钛表面诱导的巨噬细胞调控促进原骨化信号的传递

2020-05-08 MedSci原创 MedSci原创

由于生物材料诱导的免疫应答介质的调节可能是增强伤口愈合事件的潜在治疗方法,因此本研究旨在描述钛表面修饰对巨噬细胞表型和功能的影响。

由于生物材料诱导的免疫应答介质的调节可能是增强伤口愈合事件的潜在治疗方法,因此本研究旨在描述钛表面修饰对巨噬细胞表型和功能的影响。

 

将啮齿类动物的骨髓巨噬细胞极化为M1和M2表型,并在微粗糙(SLA)和亲水改性SLA(modSLA)钛圆片上进行培养。随后分别通过免疫染色和ELISA评估巨噬细胞表型和细胞因子分泌。在M1和M2巨噬细胞条件培养基中培养7天,通过RT-PCR评估了成骨细胞基因的表达。

 

ModSLA表面的M1巨噬细胞培养促进了M2型的表型,表现为明显的CD163蛋白表达、Arg1基因的表达和细胞因子的分泌,这些细胞因子在破骨细胞中显著上调了与TGF-ß/BMP信号通路和成骨细胞的相关基因的表达。相比之下,SLA表面的M2巨噬细胞培养促进了炎症表型和细胞因子谱,不利于成骨基因表达。

 

综上所述,该研究结果表明,巨噬细胞能够根据从生物材料表面接收到的信号改变或改变其表型。亲水的微粗糙钛表面形貌引起巨噬细胞表型,与炎症减少和成骨信号增强有关。

 

原始出处:

 

Stephen M Hamlet, Ryan S B Lee, et al., Hydrophilic Titanium Surface-Induced Macrophage Modulation Promotes Pro-Osteogenic Signalling. Clin Oral Implants Res. 2019 Nov;30(11):1085-1096. doi: 10.1111/clr.13522. Epub 2019 Aug 30.

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    2021-02-24 xuyu
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    2020-11-01 30397613
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    2020-06-29 小几洁
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    2020-06-10 xuyong536

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