J Endod:伴随牙周病的活髓牙在牙髓治疗不同阶段的微生物模式和内毒素以及脂膜酸水平调查

2020-04-19 lishiting MedSci原创

这篇体内研究的目的是为了评估伴随牙周病的活髓牙在牙髓治疗不同阶段的微生物模式以及脂多糖(LPS)和脂膜酸(LTA)水平。

这篇体内研究的目的是为了评估伴随牙周病的活髓牙在牙髓治疗不同阶段的微生物模式以及脂多糖(LPS)和脂膜酸(LTA)水平。

这篇临床研究纳入了10名患者。在机械化学预备前后以及根管内封药后从牙周袋(PPs)和根管(RCs)内使用无菌纸尖提取样本。采用套式聚合酶链式反应和checkerboard DNA-DNA杂交进行微生物分析。分别通过鲎阿米巴样细胞溶解物和酶联免疫吸附测定检测LPS和LTA水平。数据进行统计学分析,显著性水平设置为5%。

结果显示,通过套式聚合酶链式反应,从PPs中提取并检测到17种细菌DNA,其中6种不存在于RCs的最初样本中。通过checkerboard DNA-DNA杂交,在最初的样本中,PPs内的40个探针检测到38个,而在RCs内的40个探针检测到12个。总体上讲,牙髓治疗有效,能够改变PPs和RCs内的微生物丛。尽管在PPs中检测到2者的浓度明显高于RCs,牙髓治疗后LPS和LTA水平下降。

结论:伴随牙周炎的活髓牙其PPs和RCs内的微生物丛是多菌属的,包括革兰氏阳性、阴性以及兼性和专性厌氧菌。

原始出处

Lidiane M Louzada, Rodrigo Arruda-Vasconcelos, et al. Clinical Investigation of Microbial Profile and Levels of Endotoxins and Lipoteichoic Acid at Different Phases of the Endodontic Treatment in Teeth With Vital Pulp and Associated Periodontal Disease. J Endod., 2020 Apr 10;S0099-2399(20)30116-3. doi: 10.1016/j.joen.2020.02.005.

 

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    2021-01-04 jxrzshh
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