J Endod:使用自体DPSC和富含白细胞血小板纤维蛋白的个性化细胞疗法治疗牙髓炎:一项病例汇报

2019-02-06 lishiting MedSci原创

牙髓再生疗法作为一种全新的治疗方法已经涌现。这篇病例汇报描述的是一例使用来源于自体感染牙髓的间充质干细胞和发育成熟牙齿的富含白细胞血小板纤维蛋白(L-PRF)实施的再生细胞疗法。

牙髓再生疗法作为一种全新的治疗方法已经涌现。这篇病例汇报描述的是一例使用来源于自体感染牙髓的间充质干细胞和发育成熟牙齿的富含白细胞血小板纤维蛋白(L-PRF)实施的再生细胞疗法。

一名健康的50岁男性患者#28牙齿存在自发性疼痛,诊断为有症状的不可逆牙髓炎,需要牙髓治疗。抽提感染牙髓并将其送入一家较好的实验室进行牙髓干细胞(DPSCs)的分离和培养。L-PRF从患者血液中获取,并将其导入已预备及去感染的根管内,将扩增的DPSCs接种于血凝块内。根管颈部Biodentine (Septodont, Saint-Maur-des-Fosses, France)封闭,复合树脂充填。

随访检查在术后6个月和3年。检查内容包括根尖周放射线影像(检测尖周指数[PAI])、CBCT影像、敏感和活力测试。临床检查显示,门诊和叩诊正常。牙齿对冷刺激反应延迟,对电活力反应敏感。PAI和CBCT影像观察显示,尖周区域正常,PAI评分为1以及CBCT评分为0。活力测试呈现低血液灌注单位。

结论:这篇研究表明,采用自体DPSCs和L-PRF作为治疗成熟恒牙牙髓炎的一种选择具有潜在的应用价值。它也为设计个性化的依赖于细胞的牙髓再生疗法的临床试验铺设了道路。

原始出处:

Meza G, Urrejola D, et al. Personalized Cell Therapy for Pulpitis Using Autologous Dental Pulp Stem Cells and Leukocyte Platelet-rich Fibrin: A Case Report. J Endod. 2019 Feb;45(2):144-149. doi: 10.1016/j.joen.2018.11.009.

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    2019-08-07 jxrzshh
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    2019-02-08 gjsgj
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    2019-02-08 jiyangfei
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