J Clin Periodontol:种植体周围微生物的检测

2017-12-14 MedSci MedSci原创

比较种植体周围健康(H)和病变(P)位点的微生物组,并确定种植体周围核心微生物。

比较种植体周围健康(H)和病变(P)位点的微生物组,并确定种植体周围核心微生物。

材料和方法:使用16S rRNA测序(MiSeq,Illumina),QIIME和HOMINGS分析32 个H和35个 P位点的粘膜下微生物。使用主坐标分析(PCoA),t检验和Wilcoxon秩和检验和FDR调整来确定组间差异。确定了种植体周围核心微生物组。

结果:PCoA显示在所有分类水平上H和P之间的分配。Bacteroidetes,Spirochetes和Synergistetes在P中较高,而放线菌在H中占优势(p <.05)。Porphyromonas和Treponema在P中含量较高,而Rothia和Neisseria在H中较高(p <0.05)。核心种植体微生物组含有Fusobacterium,Parvimonas和Campylobacter sp。T.denticola, 和P.gingivalis以及F. alocis,F. fastidiosum和T. maltophilum在P组中较高(p <.05)。

结论:种植体周围炎微生物群是共生体消耗和病原体丰富的,包含原有的和新的病原体。核心种植体周围的微生物群通常含有与牙周炎症有关的属。

原始出处:

Ignacio Sanz-Martin, et al. Exploring the microbiome of healthy and diseased peri-implant sites using Illumina sequencing. Journal of Clinical Periodontology. 2017 December. doi:10.1111/jcpe.12788

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    2017-12-31 feather89
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    2018-11-03 huangdf
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