Dent Mater：不同种植体表面成骨和炎症细胞的反应

2019-02-09 MedSci MedSci原创

粗糙表面的牙科植入物可促进间充质干细胞（MSC）成骨细胞分化，且亲水性修饰诱导抗炎性巨噬细胞活化。虽然已经在体内和临床上比较了不同表面修饰对商业牙科植入物的骨整合的影响，但是对这些修饰的初始细胞反应经常被忽视。本研究旨在表征不同的市售植入物表面巨噬细胞炎症反应和MSC成骨。六种市售的粗植体[OsseoSpeed TM（Astra-Tech TM，Implant A）; Osseotite®（Bio

粗糙表面的牙科植入物可促进间充质干细胞（MSC）成骨细胞分化，且亲水性修饰诱导抗炎性巨噬细胞活化。虽然已经在体内和临床上比较了不同表面修饰对商业牙科植入物的骨整合的影响，但是对这些修饰的初始细胞反应经常被忽视。本研究旨在表征不同的市售植入物表面巨噬细胞炎症反应和MSC成骨。

六种市售的粗植体[OsseoSpeed TM（Astra-Tech TM，Implant A）; Osseotite®（Biomet 3i™，植入物B）; TiUnite™（Nobel-Biocare®，Implant C）;检查Ti-SLA，（植入物D），Roxolid（RXD-SLA，植入物E），RXD-SLActive（植入物F）（Straumann）]。将巨噬细胞和MSC直接接种在植入物上并在定制小瓶中培养。在巨噬细胞中24和48小时后测量促炎- （IL1B，IL6，IL17A，CXCL10，TNFα）和抗炎=（IL4，IL10，TGFβ1）标记物的mRNA和蛋白质水平。通过ELISA和qPCR（n = 6 /变量，ANOVA，事后Tukey HSD，α= 0.05），通过7天后碱性磷酸酶活性，骨钙蛋白和血管生成，成骨和炎症标志物评估MSC的成骨细胞分化。

结果显示，亲水性植入物F诱导从MSCs释放的最高水平的成骨因子和最低水平的促炎因子。或者，植入物A和C支持较低水平的骨生成和增加促炎因子的分泌。

总之，在本研究中，我们使用体外模型成功评估了细胞对市售临床植入物的反应差异。来自该模型的数据表明并非所有表面修饰都会产生相同的细胞反应。

原始出处：

Hotchkiss KM, Sowers KT, Olivares-Navarrete R. Novel in vitro comparative model of osteogenic and inflammatory cell response to dental implants. Dent Mater. 2019 Jan;35(1):176-184. doi: 10.1016/j.dental.2018.11.011. Epub 2018 Nov 30.

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2019-11-30 huangdf

#种植#

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2019-09-18 楚钟

#炎症细胞#

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2019-02-10 ms6279672939590805

#种植体#

32 0
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2019-02-10 zhaojie88

#成骨#

41 0
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08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 明月清辉

谢谢分享，学习了

48 0
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08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 zwjnj2

学习学习

45 0
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08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 1e145228m78(暂无匿称)

学习了，谢谢作者分享!

51 0
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08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 一天没事干

很好的学习机会

35 0
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08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 龙胆草

学习谢谢分享

50 0
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objectType=article, channel=null, level=null, likeNumber=45, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sat Feb 09 11:17:16 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360248, encodeId=b82936024891, content=学习了，谢谢作者分享!, beContent=null, objectType=article, channel=null, level=null, likeNumber=51, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mONcle9pic3xJXLtMtt5B40XXuPjn9xn1rBhrM6fHLkX1hI86ZoWoPJkgDkZuJNKkBfBHOcRd1fAdezg7JlHNOokohdAp3WkI/0, createdBy=32c81964262, createdName=1e145228m78(暂无匿称), createdTime=Sat Feb 09 10:46:41 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360246, encodeId=f98a36024690, content=很好的学习机会, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2018/04/12/cd30eefc465f70dc4dad89bd57479781.jpg, createdBy=02be2318568, createdName=一天没事干, createdTime=Sat Feb 09 10:42:21 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360233, encodeId=33163602337a, content=学习谢谢分享, beContent=null, objectType=article, channel=null, level=null, likeNumber=50, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/mStl88fu4Nd44v3xHnnictn9UpctwtH62EPQ59TMdI9lnqHMgQ16nH7GmGCfpzicsHgjibYVk6vwSpXrC86gMibRkoaIzf5HwSz7/0, createdBy=78cc1691107, createdName=龙胆草, createdTime=Sat Feb 09 08:20:51 CST 2019, time=2019-02-09, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=360224, encodeId=90433602248f, content=好好好好好好, beContent=null, objectType=article, channel=null, level=null, likeNumber=18, replyNumber=0, topicName=null, topicId=null, topicList=[], attachment=null, authenticateStatus=null, createdAvatar=https://cdnapi.center.medsci.cn/medsci/head/2017/08/29/bbc5cc0eed9b8d17623d2fd49dbdf19a.jpg, createdBy=79522135794, createdName=yjs木玉, createdTime=Sat Feb 09 05:54:39 CST 2019, time=2019-02-09, status=1, ipAttribution=)]
2019-02-09 yjs木玉

好好好好好好

18 0