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J Cell Physiol：在改性聚L-乳酸纳米纤维上脂多糖预处理骨髓间充质干细胞可增加成骨分化

2019-02-09 MedSci MedSci原创

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组织工程包括多学科，涉及使用纳米支架修复，修复和去除组织缺陷以及新组织的形成。间充质干细胞（MSCs）可以分化成多种细胞类型，它们是组织工程的有吸引力的候选者。当前研究中，研究人员使用静电纺丝工艺制备纳米纤维，聚-1-乳酸（PLLA）聚合物。用O2等离子体处理表面以增强亲水性、细胞附着、生长和分化潜力。使用脂多糖（LPS）预处理纳米支架以增强分化诱导。通过接触角测量和扫描电子显微镜表征纳米支架。使

组织工程包括多学科，涉及使用纳米支架修复，修复和去除组织缺陷以及新组织的形成。间充质干细胞（MSCs）可以分化成多种细胞类型，它们是组织工程的有吸引力的候选者。

当前研究中，研究人员使用静电纺丝工艺制备纳米纤维，聚-1-乳酸（PLLA）聚合物。用O2等离子体处理表面以增强亲水性、细胞附着、生长和分化潜力。使用脂多糖（LPS）预处理纳米支架以增强分化诱导。通过接触角测量和扫描电子显微镜表征纳米支架。使用MTT分析细胞的生长和增殖速率。定量实时聚合酶链反应和免疫细胞化学分析纳米纤维上评估培养的MSC的成骨分化，评估包括常见的成骨标志物，碱性磷酸酶活性以及钙矿物质沉积。

结果显示，在PLLA纳米支架上具有LPS的引发的MSC显著增强了MSC的增殖和成骨。此外，LPS和电纺纳米纤维的组合可以提供新的和合适的基质，以支持干细胞分化骨组织工程。

原始出处：

Kooshki H, Ghollasi M, et al., Osteogenic differentiation of preconditioned bone marrow mesenchymal stem cells with lipopolysaccharide on modified poly-l-lactic-acid nanofibers. J Cell Physiol. 2019 May;234(5):5343-5353. doi: 10.1002/jcp.26567. Epub 2018 Dec 4.

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    2019-11-07 维他命

    #CEL#

    0

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    2019-04-27 HinsMax

    #骨分化#

    0

  4. 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    2019-12-31 h.y200607_23674418

    #间充质干细#

    0

  5. 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createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sun Feb 10 20:51:15 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337541, encodeId=3f18133e5410f, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Sun Feb 10 17:42:00 CST 2019, time=2019-02-10, status=1, ipAttribution=)]
    2019-02-24 ying_wu

    #骨髓间充质干细胞#

    0

  6. 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createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sun Feb 10 20:51:15 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337541, encodeId=3f18133e5410f, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Sun Feb 10 17:42:00 CST 2019, time=2019-02-10, status=1, ipAttribution=)]
    2019-08-20 zhaozhouchifen

    #Cell#

    0

  7. 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createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sun Feb 10 20:51:15 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337541, encodeId=3f18133e5410f, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Sun Feb 10 17:42:00 CST 2019, time=2019-02-10, status=1, ipAttribution=)]
    2019-06-02 cmj8wellington

    #Physio#

    0

  8. 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createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sun Feb 10 20:51:15 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337541, encodeId=3f18133e5410f, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Sun Feb 10 17:42:00 CST 2019, time=2019-02-10, status=1, ipAttribution=)]
    2019-02-13 yjs木玉

    学习学习学习学习

    0

  9. 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createdAvatar=https://wx.qlogo.cn/mmopen/Q3auHgzwzM6DGOvNrRdcib3UmzMgpEQpl0ENSJScphsFtdgfibXX1g1IuEYGCjP23UqCd52TxTpSZibtPiclyusaHicnNXd78alaia19Qy1nAFo18/0, createdBy=50381491660, createdName=zwjnj2, createdTime=Sun Feb 10 20:51:15 CST 2019, time=2019-02-10, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1337541, encodeId=3f18133e5410f, content=<a href='/topic/show?id=863f5409610' target=_blank style='color:#2F92EE;'>#成骨分化#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=26, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54096, encryptionId=863f5409610, topicName=成骨分化)], attachment=null, authenticateStatus=null, createdAvatar=http://thirdwx.qlogo.cn/mmopen/vi_32/DYAIOgq83eqySEUYKeYDjXJMYMrtscBNkpAnLfQZgDcdkEratcJPqS7ARJOXqllZQhCSFicErlBFbIywuoiaP0LQ/132, createdBy=012d2500132, createdName=ms5880526374914242, createdTime=Sun Feb 10 17:42:00 CST 2019, time=2019-02-10, status=1, ipAttribution=)]
    2019-02-10 zwjnj2

    学习学习努力学习

    0

  10. 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    2019-02-10 ms5880526374914242

    #成骨分化#

    0

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