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CLIN CHEM LAB MED：基于血型糖蛋白A的红细胞排除法，用于流式细胞术分析柠檬酸化全血中血小板糖蛋白的表达

2020-11-30 MedSci原创 MedSci原创

血；血小板糖蛋白
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通过流式细胞仪分析血小板糖蛋白（GP）的表达被用于诊断相关的GP相关的血小板减少症。尽管富含血小板的血浆可用于目标事件的明确识别，但这种策略对小样本量或血小板计数低和/或血小板过多的患者不可行。

通过流式细胞仪分析血小板糖蛋白（GP）的表达被用于诊断相关的GP相关的血小板减少症。尽管富含血小板的血浆可用于目标事件的明确识别，但这种策略对小样本量或血小板计数低和/或血小板过多的患者不可行。但是，由于难以将血小板与红细胞（RBC）区分开来，因此妨碍了全血（WB）的使用。为了避免这些限制，我们评估了RBC门控策略的可行性。

除了血小板GPIb、GPIIa/IIIa和p -选择素(CD62P)，柠檬酸全血(CWB)标本还对红细胞特异性糖素A (CD235a)进行了染色。cd235a阴性的血小板事件通过正向/侧散点特征进一步鉴别，血小板GP表达分析相对于平行处理的健康对照样本。

建立的参考区间可以明确分别在确诊为Glanzmann血栓性贫血或Bernard-Soulier综合征患者样本中GPIIb/IIIa-或GPIb表达模式降低。可以表明，对2500个血小板事件的分析足以进行可靠的GP表达分析，使所提出的方法适用于低血小板计数的样本。

这项研究证明了基于cd235的RBC排除用于CWB中血小板GP表达分析的可行性。与直接染色血小板特异性抗原进行目标识别相比，这种间接门控的方法通常适用于任何潜在的血小板GP表达不足。

原始出处：

Christina Berens，Johannes Oldenburg，Glycophorin A-based exclusion of red blood cells for flow cytometric analysis of platelet glycoprotein expression in citrated whole blood

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    2020-12-02 kord1982

    #红细胞#

    0

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    2020-12-02 w984448tii

    #流式细胞术#

    0

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    2020-12-02 ms5236388012055669

    #柠檬酸#

    0

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    2020-12-02 ms3994565386320060

    #Med#

    0

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    2020-12-02 chentianping

    #糖蛋白#

    0