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Stem Cells Int：煅烧牙粉的条件培养基通过MAPK信号通路促进人牙髓干细胞的成骨和牙源性分化

2019-04-29 不详 网络

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既往研究已经证实煅烧的牙粉（CTP）是一种同种异体仿生矿化材料，可在高温下获得并促进新骨形成。本研究旨在研究煅烧牙粉（CTP-CM）条件培养基对人牙髓干细胞（hDPSCs）的成骨和牙源性分化的影响以及所涉及的潜在机制。首先，ALP活性测定确定200μg/mL是用于以下实验的CTP-CM的最佳浓度。如CCK-8和FCM分析所示，CTP-CM对hDPSC的增殖没有明显影响。与对照组相比，CTP-CM诱

既往研究已经证实煅烧的牙粉（CTP）是一种同种异体仿生矿化材料，可在高温下获得并促进新骨形成。本研究旨在研究煅烧牙粉（CTP-CM）条件培养基对人牙髓干细胞（hDPSCs）的成骨和牙源性分化的影响以及所涉及的潜在机制。

首先，ALP活性测定确定200μg/mL是用于以下实验的CTP-CM的最佳浓度。如CCK-8和FCM分析所示，CTP-CM对hDPSC的增殖没有明显影响。与对照组相比，CTP-CM诱导的hDPSC中基因和蛋白质（DSPP/DSPP，RUNX2/RUNX2，OCN/OCN，OSX/OSX，OPN/OPN，ALP/ALP和COL-1/COL-1）表达水平在第3天或第7天均增加，显示CTP-CM对hDPSCs的骨/牙源性分化的正调节。机制上，在CTP-CM处理后MAPK信号传导通路被激活，并且鉴定了靶向MAPK的抑制剂，其削弱了CTM-CM对hDPSC的定向分化的影响。

总之，这些发现或可用于牙科再生的干细胞疗法。

原始出处：

Wu J, Li N, et al., The Conditioned Medium of Calcined Tooth Powder Promotes the Osteogenic and Odontogenic Differentiation of Human Dental Pulp Stem Cells via MAPK Signaling Pathways. Stem Cells Int. 2019 Mar 19;2019:4793518. doi: 10.1155/2019/4793518. eCollection 2019.

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    2019-07-25 zhaozhouchifen

    #Cell#

    0

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    2020-02-16 yykkxiaodou

    #stem cells#

    0

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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-08-09 hb2008ye

    #stem cell#

    0

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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-08-27 柳叶一刀

    #牙髓#

    0

  5. 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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-06-15 维他命

    #CEL#

    0

  6. 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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2020-03-11 lidong51

    #MAPK#

    0

  7. 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href='/topic/show?id=8cc416e78f3' target=_blank style='color:#2F92EE;'>#STEM#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16778, encryptionId=8cc416e78f3, topicName=STEM)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d1067833268, createdName=12498bb3m92(暂无昵称), createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1539740, encodeId=27fb1539e4077, content=<a href='/topic/show?id=c61a2e315da' target=_blank style='color:#2F92EE;'>#信号通路#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27315, encryptionId=c61a2e315da, topicName=信号通路)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b12d13147749, createdName=wgx310, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-05-01 zhaojie88

    #成骨#

    0

  8. 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channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11284, encryptionId=c95711284ca, topicName=MAPK)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21d332479882, createdName=lidong51, createdTime=Wed Mar 11 06:10:00 CST 2020, time=2020-03-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352116, encodeId=1d4d1352116e4, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1482249, encodeId=f9ad1482249a8, content=<a href='/topic/show?id=8cc416e78f3' target=_blank style='color:#2F92EE;'>#STEM#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=24, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16778, encryptionId=8cc416e78f3, topicName=STEM)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=d1067833268, createdName=12498bb3m92(暂无昵称), createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1539740, encodeId=27fb1539e4077, content=<a href='/topic/show?id=c61a2e315da' target=_blank style='color:#2F92EE;'>#信号通路#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=27315, encryptionId=c61a2e315da, topicName=信号通路)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=b12d13147749, createdName=wgx310, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-05-01 12498bb3m92(暂无昵称)

    #STEM#

    0

  9. [GetPortalCommentsPageByObjectIdResponse(id=1871258, encodeId=bf6718e12584c, content=<a href='/topic/show?id=fd764459a8' target=_blank style='color:#2F92EE;'>#Cell#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4459, encryptionId=fd764459a8, topicName=Cell)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=524d95, createdName=zhaozhouchifen, createdTime=Thu Jul 25 14:10:00 CST 2019, time=2019-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1794970, encodeId=19551e949708d, content=<a href='/topic/show?id=46e716e82a6' target=_blank style='color:#2F92EE;'>#stem cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16782, encryptionId=46e716e82a6, topicName=stem cells)], attachment=null, authenticateStatus=null, createdAvatar=, 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channel=null, level=null, likeNumber=28, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=11284, encryptionId=c95711284ca, topicName=MAPK)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=21d332479882, createdName=lidong51, createdTime=Wed Mar 11 06:10:00 CST 2020, time=2020-03-11, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1352116, encodeId=1d4d1352116e4, content=<a href='/topic/show?id=e95954093cd' target=_blank style='color:#2F92EE;'>#成骨#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=25, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=54093, encryptionId=e95954093cd, topicName=成骨)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=4c37265, createdName=zhaojie88, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1482249, encodeId=f9ad1482249a8, content=<a 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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-05-01 wgx310

    #信号通路#

    0

  10. [GetPortalCommentsPageByObjectIdResponse(id=1871258, encodeId=bf6718e12584c, content=<a href='/topic/show?id=fd764459a8' target=_blank style='color:#2F92EE;'>#Cell#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=23, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=4459, encryptionId=fd764459a8, topicName=Cell)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=524d95, createdName=zhaozhouchifen, createdTime=Thu Jul 25 14:10:00 CST 2019, time=2019-07-25, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1794970, encodeId=19551e949708d, content=<a href='/topic/show?id=46e716e82a6' target=_blank style='color:#2F92EE;'>#stem cells#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=35, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=16782, encryptionId=46e716e82a6, topicName=stem cells)], attachment=null, authenticateStatus=null, createdAvatar=, 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time=2019-05-01, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1544903, encodeId=a1d915449036c, content=<a href='/topic/show?id=f5576e67147' target=_blank style='color:#2F92EE;'>#牙髓干细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=41, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=67671, encryptionId=f5576e67147, topicName=牙髓干细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=841913671773, createdName=ms6519009802973839, createdTime=Wed May 01 09:10:00 CST 2019, time=2019-05-01, status=1, ipAttribution=)]
    2019-05-01 ms6519009802973839

    #牙髓干细胞#

    0

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