Clinica Chimica Acta：快速液相色谱-串联质谱联用技术测定人体内的长链脂肪酸，并建立中国人的参考区间

2019-09-21 gladiator MedSci原创

长链脂肪酸<span lang="EN-US" style="font-size:12.0pt;mso-bidi-font-size:14.0pt;font-family:"Calibri","sans-serif"; mso-fareast-font-family:宋体;mso-bidi-font-family:"Times New Roman

长链脂肪酸(VLCFAs)是过氧化物酶体疾病的标志物，包括六士古酸、四环酸和二十二酸，其异常积累需要及时发现，以便获得及时有效地治疗。本研究旨在建立并验证一种基于液相色谱-串联质谱(LC-MS/MS)的方法，同时对VLCFAs进行定量，并在中国人个体间提供参考区间，并对妊娠期VLCFAs进行定量，探索血浆和羊水之间的潜在联系。

研究人员采用盐酸水浴培养法和液-液萃取法提取分析物。根据临床和实验室标准研究所（CLSI）的指南评估方法线性，检测/定量，精度，交叉污染等。 VLCFAs表现出良好的再现性，基于低内运行系数的变化（CVS）和总的CV和线性的相关系数均大于0.99。 C22:0, C24:0及C26:0的参考区间分别为 32.0–73.4 μmol/L, 30.3–72.0 μmol/L和0.20–0.71 μmol/L，C24:0/C22:0和C26:0/C22:0比值分别为0.75–1.28 和0.005–0.0139，同一孕妇的血浆和羊水在妊娠中期无明显相关性。

本研究提出了一种简便、高效、准确、稳定的LC-MS/MS方法，可以同时检测C22:0、C24:0和C26:0，且无需衍生化；可用于建立中国个体间的参考区间，并具有诊断和临床应用价值。

原始出处：

Danchen Wang，Songlin Yu，Rapid liquid chromatography-tandem mass spectrometry to determine very-long-chain fatty acids in human and to establish reference intervals for the Chinese population

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2019-10-25 丁鹏鹏

#测定#

29 0
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2019-11-06 w984455tii

#长链脂肪酸#

41 0
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2020-02-09 windight

#CTA#

37 0
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2019-09-23 12498dabm81暂无昵称

#参考区间#

33 0
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2019-09-23 jeanqiuqiu

#质谱#

33 0

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Clinica Chimica Acta：快速液相色谱-串联质谱联用技术测定人体内的长链脂肪酸，并建立中国人的参考区间

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