ARD:外周血巨核细胞在类风湿性关节炎、系统性红斑狼疮和原发性干燥综合征中扩增

2021-09-19 MedSci原创 MedSci原创

类风湿性关节炎 (RA)、系统性红斑狼疮 (SLE) 和原发性干燥综合征(pSS)具有许多相似的临床和血清学特征。外周血单核细胞 (PBMC) 是常见的浸润特异靶器官的免疫细

   类风湿性关节炎 (RA)、系统性红斑狼疮 (SLE) 和原发性干燥综合征(pSS)具有许多相似的临床和血清学特征。外周血单核细胞 (PBMC) 是常见的浸润特异靶器官的免疫细胞起源。然而,受核心转录因子 (TF) 调控的原初免疫细胞仍未知。本研究的目的是发现这些自身免疫病(AD)共同的转录谱和外周血中免疫细胞的组成成分

    汕头大学医学院Guohong Zhang团队分析了来自119个未经治疗患者(41 RA38 pSS28 SLE 12个多重自​​身免疫)和20个健康对照PBMCRNA-seq数据,做了生物过程和转录因子富集和基于反卷积的免疫细胞类型分析。单细胞RNA测序(scRNA-seq)和流式细胞术用于进一步定义PBMC中的免疫细胞亚群。

     结果显示RASLEpSS 和多发性自身免疫病具有相似的转录谱以及共同的与核小体组装和止血相关的基因表达特征。不同的TF合集和基因调控网络主要富集于造血过程。上调的细胞谱系特异性TFs,包括PBX1GATA1TAL1 GFI1B显示巨核细胞(MK)的强基因表达特征。基于基因表达的细胞类型富集显示MK成分升高,CD41b+CD42b+ CD41b+CD61+ MK亚群增多,并通过流式细胞术得到证实。scRNA-seq数据中,MKsPBX1/GATA1/TAL1pre-T-细胞抗原受体基因PTCRA定义。在MK中观察到细胞异质性独特的抗原提呈功能性富集的亚群

结论MK扩增的鉴定提供了RASLEpSS 和多发性自身免疫病外周免疫细胞图谱的新见解。使MK扩增的异常调控机制可能促进了这些AD的发病

 

出处:

Wang Y, Xie X, Zhang C, et al. Rheumatoid arthritis, systemic lupus erythematosus and primary Sjögren’s syndrome shared megakaryocyte expansion in peripheral blood. Annals of the Rheumatic Diseases. Published Online First: 30 August 2021. doi: 10.1136/annrheumdis-2021-220066

 

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style='color:#2F92EE;'>#巨核细胞#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=116, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=48201, encryptionId=af9f48201a9, topicName=巨核细胞)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=f3d78715376, createdName=liuquan, createdTime=Wed Sep 22 02:52:13 CST 2021, time=2021-09-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1531076, encodeId=2a4c15310e629, content=<a href='/topic/show?id=06413e05695' target=_blank style='color:#2F92EE;'>#原发性干燥综合征#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=138, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37056, encryptionId=06413e05695, topicName=原发性干燥综合征)], attachment=null, authenticateStatus=null, createdAvatar=null, createdBy=2aa512204505, createdName=dinorsor528, createdTime=Wed Sep 22 02:52:13 CST 2021, time=2021-09-22, status=1, ipAttribution=)]
    2021-09-22 lmm397
  4. 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  5. [GetPortalCommentsPageByObjectIdResponse(id=1846989, encodeId=025918469899e, content=<a href='/topic/show?id=d3a33e0306d' target=_blank style='color:#2F92EE;'>#原发性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37030, encryptionId=d3a33e0306d, topicName=原发性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8e4c53, createdName=jiyangfei, createdTime=Wed Aug 31 23:52:13 CST 2022, time=2022-08-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2017295, encodeId=cb5c201e295f4, content=<a href='/topic/show?id=9760e69058c' target=_blank style='color:#2F92EE;'>#系统性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76905, encryptionId=9760e69058c, topicName=系统性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b9b368, 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  6. [GetPortalCommentsPageByObjectIdResponse(id=1846989, encodeId=025918469899e, content=<a href='/topic/show?id=d3a33e0306d' target=_blank style='color:#2F92EE;'>#原发性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37030, encryptionId=d3a33e0306d, topicName=原发性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8e4c53, createdName=jiyangfei, createdTime=Wed Aug 31 23:52:13 CST 2022, time=2022-08-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2017295, encodeId=cb5c201e295f4, content=<a href='/topic/show?id=9760e69058c' target=_blank style='color:#2F92EE;'>#系统性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76905, encryptionId=9760e69058c, topicName=系统性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b9b368, 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encodeId=c35f1432150eb, content=<a href='/topic/show?id=f9b9e9124a8' target=_blank style='color:#2F92EE;'>#综合征#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79124, encryptionId=f9b9e9124a8, topicName=综合征)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Wed Sep 22 02:52:13 CST 2021, time=2021-09-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1436296, encodeId=976d1436296d9, content=<a href='/topic/show?id=22f8e71238d' target=_blank style='color:#2F92EE;'>#红斑#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77123, encryptionId=22f8e71238d, topicName=红斑)], attachment=null, 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  7. [GetPortalCommentsPageByObjectIdResponse(id=1846989, encodeId=025918469899e, content=<a href='/topic/show?id=d3a33e0306d' target=_blank style='color:#2F92EE;'>#原发性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=73, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=37030, encryptionId=d3a33e0306d, topicName=原发性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=8e4c53, createdName=jiyangfei, createdTime=Wed Aug 31 23:52:13 CST 2022, time=2022-08-31, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=2017295, encodeId=cb5c201e295f4, content=<a href='/topic/show?id=9760e69058c' target=_blank style='color:#2F92EE;'>#系统性#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=103, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=76905, encryptionId=9760e69058c, topicName=系统性)], attachment=null, authenticateStatus=null, createdAvatar=, createdBy=2b9b368, 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encodeId=c35f1432150eb, content=<a href='/topic/show?id=f9b9e9124a8' target=_blank style='color:#2F92EE;'>#综合征#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=68, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=79124, encryptionId=f9b9e9124a8, topicName=综合征)], attachment=null, authenticateStatus=null, createdAvatar=https://wx.qlogo.cn/mmopen/aLGWoFXAyMbIu3qymFOyheQLjPSX3OUs5GmkyBlcCOwTPIeq3why9NGibxxUqYo6hcx8qZLHZFgNPnBK1yzWeOFpyg2OnWOt0/0, createdBy=fa4716, createdName=zhouqu_8, createdTime=Wed Sep 22 02:52:13 CST 2021, time=2021-09-22, status=1, ipAttribution=), GetPortalCommentsPageByObjectIdResponse(id=1436296, encodeId=976d1436296d9, content=<a href='/topic/show?id=22f8e71238d' target=_blank style='color:#2F92EE;'>#红斑#</a>, beContent=null, objectType=article, channel=null, level=null, likeNumber=67, replyNumber=0, topicName=null, topicId=null, topicList=[TopicDto(id=77123, encryptionId=22f8e71238d, topicName=红斑)], attachment=null, 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    2021-09-22 zhouqu_8
  8. 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  9. 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  10. 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